An optimized semi-defined medium for p-coumaric acid production in extractive fermentation. (November 2022)

• Record Type: Journal Article
• Authors: Combes, Jeanne; Imatoukene, Nabila; Couvreur, Julien; Godon, Blandine; Fojcik, Clémentine; Allais, Florent; Lopez, Michel
• Is Part Of: Process biochemistry. Volume 122(2022)Supplement Part 2
• Page Start: 357
• Page End: 362
• Publication Date: 2022-11
• Subjects: Design of experiments -- p-coumaric acid -- Medium optimization -- Biphasic fermentation -- Saccharomyces cerevisiae; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2022.10.021
• Languages: English
• ISSNs: 1359-5113

Abstract

Abstract: In this study, the definition and optimization of a medium for cost-effective heterologous production as well as efficient recovery of the plant secondary metabolite p -coumaric acid ( p -CA) was performed. Experimental designs were carried out to this end. First, a Plackett Burman design was used to choose the important factors that affect p -CA production. Then, a central composite face centered design was used to find the optima of the two major parameters, C/N ratio and pH. Finally, a four-component optimized semi-defined (OSD) medium was developed and experimented in both biphasic and control batch fermentations. In comparison to the results of previous biphasic fermentations work using a classic defined medium (a 2X Yeast Nitrogen Base medium without Amino Acids), OSD medium was 10 times less expensive per litre. At pH 4.5, the final p -CA production in OSD medium was approximately 61 % of the p -CA content reached in the classic defined medium. At pH 6.0, the production of p -CA in OSD medium was 55 % of the p -CA reached in the YNB medium. Graphical Abstract: ga1 Highlights: High C/N ratio and controlled pH of 6.0 promote p -coumaric acid ( p -CA) production. Extractive biphasic fermentation improved yeast growth compared to control. Optimized semi-defined (OSD) medium was 10 times cheaper than 2X YNB medium. OSD medium in biphasic fermentation at pH 4.5 prevented p -CA decarboxylation.