Vernodalin regulated the NF-κβp65 signaling in inflammation of lipopolysaccharide -induced sepsis rats. (November 2022)

• Record Type: Journal Article
• Authors: Duan, Hong-Dan; Cheng, Zhong-Feng; Zhu, Jun-Bo; Hu, Rui; Li, Xiao-Yan
• Is Part Of: Process biochemistry. Volume 122(2022)Supplement Part 2
• Page Start: 323
• Page End: 331
• Publication Date: 2022-11
• Subjects: SIRS -- Cytokines -- Oxidative stress -- Antioxidant -- Vernodalin; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2022.10.004
• Languages: English
• ISSNs: 1359-5113

Abstract

Abstract: Sepsis, systemic inflammatory responses in western medicine stimulated by infections various sources and traditionally in Chinese medicine belong the poison heat syndrome. Vernodalin (VN) is a well-known cytotoxic sesquiterpene that has been renowned as anti-cancer, anti-inflammatory, and anti-bacterial properties. However, the molecular mechanism of VN on sepsis has not been studied. Therefore, we investigated the anti-inflammatory and antioxidant potential of VN against LPS-prompted sepsis in rats and also assess the impact of the NF-κBp65 signaling cascade. Rats were alienated into four groups: Group-I normal control, given 1 ml DMSO; while group-II rats were injected with LPS (100 μg/kg b.w., i.p.); Group-III rats were LPS (100 μg/kg b.w., i.p.) + VN (50 mg/kg b.w. in DMSO); Group- IV rats were given VN (50 mg/kg b.w. in DMSO) alone. We examined hepatic toxic markers, inflammatory cytokines, antioxidants, histopathology, and western blot analysis. Inflammatory markers, oxidative stress, and toxicity markers were significantly (p < 0.05) enhanced in the LPS-persuaded rats, whereas VN significantly (p < 0.05) prevented oxidative stress, lipid peroxides, toxic markers, pro-inflammatory cytokines, histological changes, and thereby multi-organ damage. VN significantly (p < 0.05) suppressed the NF-κβp65 signaling pathway and inhibited the Iκβα degradation. Thus, VN exerts a promising anti-septic natural agent for LPS-induced sepsis rats. Graphical Abstract: ga1 Highlights: Vernodalin (VN) is cytotoxic sesquiterpene possess numerous biological activities. Anti-inflammatory potential of VN validated against LPS-prompted sepsis rats model. VN prevented oxidative stress, toxic markers, pro-inflammatory cytokines. VN suppressed the NF-κβp65 signalling pathway and prevented Iκβα degradation. VN act as promising anti-septic natural agent for treating LPS-induced sepsis rats.