A simple method for analysis of vitamin A palmitate in fortified cereal products using direct solvent extraction followed by reversed-phase HPLC with UV detection. (15th March 2023)

• Record Type: Journal Article
• Authors: Van Wayenbergh, Eline; Verheijen, Jolien; Langenaeken, Niels A.; Foubert, Imogen; Courtin, Christophe M.
• Is Part Of: Food chemistry. Volume 404:Part A(2023)
• Publication Date: 2023-03-15
• Subjects: Retinyl palmitate -- HPLC-UV -- Direct solvent extraction -- Cereal products; α separation factor -- Ac acetone -- Chl chloroform -- HPLC high-performance liquid chromatography -- k retention factor -- LOD limit of detection -- LOQ limit of quantification -- MeOH methanol -- MTBE methyl tert-butyl ether -- R2 coefficient of determination -- RA retinyl acetate -- RP retinyl palmitate -- Rs resolution factor -- RSD relative standard deviation -- t0 dead time -- tR retention time -- W peak width at the baseline; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134584
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: A method for the quantification of retinyl palmitate using HPLC-UV was developed. The HPLC method had a low LOD (0.01 µg/mL) and LOQ (0.03 µg/mL). Vitamin A was completely extracted from simple matrices using acetone/methanol. Vitamin A was efficiently extracted from bread and cookies with chloroform/methanol. The newly developed procedure and the standard procedure yielded equivalent results. Abstract: Vitamin A is generally analysed using a time-consuming and possibly detrimental saponification step, followed by extraction and HPLC analysis. We here developed a new method to analyse retinyl palmitate (RP) (also known as vitamin A palmitate) without the need for saponification and validated it in model systems consisting of RP, soy oil and wheat bran, and in RP-fortified cereal products. Two direct solvent extraction protocols using acetone/methanol (7/3, v/v) or chloroform/methanol (1/1, v/v) were tested. After extraction, RP was quantified by reversed-phase HPLC with UV detection. The HPLC method had low limits of detection (0.01 µg/mL) and quantification (0.03 µg/mL). Both extraction protocols showed a good recovery (88–105 %) and intra-and inter-day precision (<5%) for RP extraction from the model systems. The obtained results corresponded to results obtained using a golden standard approach. For complex food matrices like bread and cookies, the chloroform/methanol extraction protocol showed the best performance characteristics.