Facile synthesis of novel helical imprinted fibers based on zucchini-derived microcoils for efficient recognition of target protein in biological sample. (15th March 2023)

• Record Type: Journal Article
• Authors: Xu, Xinya; Xie, Yundong; Guo, Pengqi; Shi, Yongheng; Sun, Meng; Zhou, Jing; Wang, Chuan; Han, Chaojun; Liu, Jiping; Li, Ting
• Is Part Of: Food chemistry. Volume 404:Part B(2023)
• Publication Date: 2023-03-15
• Subjects: BR bromelain -- BSA bovine serum albumin -- Cyt C cytochrome C -- DA dopamine -- DOPAC 3, 4-dihydroxyphenylacetic acid -- FT-IR Fourier transform infrared spectroscopy -- HCF helical carbon fibers -- HNIFs nonimprinted polymers -- HPIFs helical protein imprinted fibers -- HSA human serum albumin -- IF imprinting factor -- Lyz lysozyme -- MIPs molecularly imprinted polymers -- Qmax the largest adsorption amount -- SC selectivity coefficient -- SEM scanning electron microscopy -- TGA thermogravimetric analysis -- XPS X-ray photoelectron spectroscopy; Protein recognition -- Helical protein imprinted fibers -- Zucchini-derived microcoils -- Biological application; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134645
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Novel helical protein imprinted fibers (HPIFs) were fabricated. Zucchini-derived microcoils were used as carriers in imprinting technique, firstly. Dopamine and 3, 4-dihydroxyphenylacetic acid were chosen as bifunctional monomers. HPIFs exhibited excellent adsorption performance and satisfactory practicability. Abstract: Highly selective recognition and purification of target proteins from complex biological matrices remains a challenging subject in natural and life sciences. Compared with natural recognition receptors, artificial imprinted polymers are an ideal alternative candidate. In this study, we report a novel method to prepare helical protein imprinted fibers (HPIFs) with zucchini-derived microcoils as a carrier, firstly. Inspired by the self-polymerization of adhesive proteins in mussels, dopamine and 3, 4-dihydroxyphenylacetic acid were chosen as bifunctional monomers for the first time to form a biocompatible imprinted layer. The chemical/physical properties and recognition performance of HPIFs were studied in a series of experiments. Additionally, the practicability of HPIFs was verified by specifically recognizing target protein in complex egg white sample. The one-step synthesis process and excellent binding performance of HPIFs make them a promising material for protein recognition and purification, and endow HPIFs with potential application value in the food, chemical and pharmaceutical fields.