Metabolite profiling of Nymphaea rubra (Burm. f.) flower extracts using cyclic ion mobility–mass spectrometry and their associated biological activities. (15th March 2023)

• Record Type: Journal Article
• Authors: Naznin, Marufa; Badrul Alam, Md; Alam, Rafiqul; Islam, Syful; Rakhmat, Sultonov; Lee, Sang-Han; Kim, Sunghwan
• Is Part Of: Food chemistry. Volume 404:Part A(2023)
• Publication Date: 2023-03-15
• Subjects: Antioxidant -- High-resolution mass spectroscopy -- Nymphaea rubra -- α-Glucosidase; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134544
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Identification of 138 phytoconstituents in Nymphaea rubra flower (NRF) with Q-cIM-TOF. First time reports of virganin, lactucopicrin methylester, and epitheaflavic acid in NRF. Four naphthopyrones were tentatively identified for the first time in the plant kingdom. NRF ethyl acetate extract inhibits α-glucosidase enzyme (IC50 : 2.48 ± 0.02 μg/mL). NRF is a potential source of phytochemicals for diabetes management. Abstract: Nymphaea rubra flowers (NRF) are widely used as a food and in folk medicine throughout the subtropical regions due to their health-promoting characteristics. This study characterized the phytochemical composition of various extracts/fractions of NRF by establishing a quadrupole–cyclic ion mobility–time-of-flight (Q-cIM-TOF) mass spectrometry method in both positive and negative electrospray ionization modes. Over 100 phytoconstituents were tentatively identified, among which 53 phytochemicals belonging to phenolic acids, tannins, flavonoids, terpenoids, alkaloids, xanthones, and naphthopyrones have never been documented in NRF before. Moreover, the ethyl acetate fraction of NRF demonstrated strong antioxidant potential (IC50 : 9.21 ± 0.47 μg/mL in DPPH assay and 13.65 ± 0.03 μg/mL in ABTS assay) and tyrosinase, α-glucosidase, and elastase inhibition (IC50 : 10.58 ± 0.20, 2.48 ± 0.02, and 38.15 ± 0.25 μg/mL, respectively). The findings highlight the value of NRF as a source of functional components and broaden its potential applications in the food and nutraceutical industries.