An ultra-sensitive photothermal lateral flow immunoassay for 17β-estradiol in food samples. (15th March 2023)

• Record Type: Journal Article
• Authors: Zhang, Yi; Yang, Han-Jie; Xu, Zhenghua; Liu, Xiao; Zhou, Jie; Qu, Xu-Fang; Wang, Wen-Long; Feng, Yongwei; Peng, Chifang
• Is Part Of: Food chemistry. Volume 404:Part A(2023)
• Publication Date: 2023-03-15
• Subjects: 17β-estradiol -- Lateral flow immunoassay -- Photothermal -- Black phosphorus; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134482
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: An ultra-sensitive photothermal lateral flow immunoassay strip (LFIA) was established for the detection of 17β-estradiol (E2 ) in food samples. Black phosphorus-gold nanocomposites modified with anti-E2 antibody were applied as photothermal probe which exhibited high photothermal conversion efficiency and achieved high signal-to-noise ratio. The LOD of this established photothermal LFIA was 0.05 ng mL −1, over 100-fold more sensitive than visual LFIA, and a linear range of 3 orders of magnitude from 0.05 ng mL -1 to 50 ng mL -1 was achieved. Abstract: Hormone residues in food and drinking water endanger human health, therefore, on-site analysis techniques of superior performance are important for monitoring this risk. In this study, an ultra-sensitive photothermal lateral flow immunoassay (LFIA) for quantification of 17β-estradiol (E2 ) has been developed. Anti-E2 antibody modified black phosphorus-Au (BP-Au) nanocomposite was developed as a photothermal contrast signal probe and the temperature at test-zone was recorded with an infrared camera. Under the irradiation of 808 nm laser at test-zone, it gave temperatures negatively related to the concentrations of E2 in samples. Under optimal detecting conditions, the developed photothermal LFIA exhibited a limit of detection of 50 pg mL −1, over 100-fold more sensitive than visual LFIA, and a linear range of 3 orders of magnitude. This method has been successfully applied to water, milk, and milk powder samples.