A TMT-based proteomic approach for investigating the effect of electron beam irradiation on the textural profiles of Litopenaeus vannamei during chilled storage. (15th March 2023)

• Record Type: Journal Article
• Authors: Wang, Haoran; Suo, Ran; Liu, Xingbo; Wang, Yangyang; Sun, Jianfeng; Liu, Yaqiong; Wang, Wenxiu; Wang, Jie
• Is Part Of: Food chemistry. Volume 404:Part A(2023)
• Publication Date: 2023-03-15
• Subjects: Litopenaeus vannamei -- Electron beam irradiation -- Proteomics -- Texture -- Refrigeration; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134548
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Effect of EBI on textural profiles of shrimp was studied by TMT-based proteomics. Potential protein markers for textural traits of irradiated shrimp were identified. A suitable dose of 5 kGy was suggested for irradiation of shrimp. Abstract: To elucidate the effect of electron beam irradiation (EBI) on the textural quality of Litopenaeus vannamei, the tandem-mass-tag labeled proteomic method was conducted to illustrate the protein changes in shrimp muscle. The results suggested that shrimp irradiated with 5 kGy exhibited optimum textural traits of hardness, springiness, and chewiness. In total, 486 proteins were identified as differentially abundance proteins (DAPs) in multiple comparison groups. Bioinformatics analysis revealed that most of DAPs participated in cellular process, binding, and catalytic. etc. Various signaling pathways, such as RNA transport and oxidative phosphorylation, were notably enriched by DAPs. The correlation analysis indicated that some DAPs such as Myosin-XVIIIa, projectin, and beta-thymosin 3 were remarkably correlated with the textural properties, which could be proposed as potential biomarkers to assess the irradiation-induced textural variation in shrimp. This study provided an insightful understanding at the protein level to improve the application of EBI to shrimp preservation.