Extractive fermentation for the production and partitioning of lipase and citric acid by Yarrowia lipolytica. (November 2022)

• Record Type: Journal Article
• Authors: Santos, Ariane Gaspar; Buarque, Filipe Smith; Ribeiro, Bernardo Dias; Coelho, Maria Alice Zarur
• Is Part Of: Process biochemistry. Volume 122(2022)Supplement Part 1
• Page Start: 374
• Page End: 385
• Publication Date: 2022-11
• Subjects: ATPS aqueous two-phase system -- CT and CB, concentration in the top phase and bottom phase, respectively -- FDA food and drug administration -- GRAS generally regarded as safe -- KL partition coefficient of lipase -- KCA, partition coefficient of citric acid -- KP, partition coefficient of protein -- KAE, partition coefficient of specific lipase activity -- kLa volumetric coefficient oxygen transfer coefficient -- LLE liquid-liquid extraction -- ηT and ηB, recovery of the components in the top phase and bottom phase, respectively -- PEG polyethylene glycol -- p-NFL p-nitrophenyl laurate -- YPD yeast extract, peptone, dextrose -- VT and VB, volume corresponding of top phase and bottom phase, respectively; Downstream process -- Extractive fermentation -- ATPS -- And Yarrowia lipolytica; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2022.09.011
• Languages: English
• ISSNs: 1359-5113

Abstract

Abstract: Extractive fermentation is an efficient downstream process to segregate the desired product in a fermentative process. Here, an integrated extractive fermentation process with an aqueous two-phase system (ATPS) was applied to evaluate the lipase and citric acid production by Yarrowia lipolytica. For this purpose, the influences of polyethylene glycol (PEG) molecular weights, different salts and different concentrations as well as the pH of the potassium phosphate buffer were studied. Citric acid preferentially migrated to the salt-rich phase, whereas lipase mostly partitioned to the PEG-rich phase. The PEG2000 (30 wt%) + salt (7 wt%) system provided higher cell growth and citric acid production, whereas the PEG2000/Na2 SO4 ATPS showed a better recovery yield (79.75%) for citric acid. The increase in PEG molecular weight reduced lipase production and the partition coefficient (KL ). In the PEG 2000/KH2 PO4 -K2 HPO4 (pH 7) system, a lipase production of 634.32 U L −1 and KL = 2.22 was obtained. Lipase production kinetics in an Erlenmeyer (10 mL) and a bioreactor (1.5 L) demonstrated that in the ATPS, the fermentation medium caused a delay in the growth curve and a peak in lipase production, of which the latter allowed for a reduction in the dissolved oxygen concentration, limiting cell metabolism. Graphical Abstract: ga1 Highlights: Lipase and citric acid production by Y. lipolytica using extractive fermentation. Citric acid migrated preferentially to the salt-rich phase. Lipase partitioned mostly to the PEG-rich phase. PEG2000/KH2 PO4 -K2 HPO4 ATPS showed a lipase production of 634.32 U L −1 and KL = 2.22. The kinetics in the miniaturised system and bioreactor showed an extensive lag phase.