Development of a three-dimensional graphene-based photoelectrochemical biosensor and its use for monitoring lipase activity. (1st December 2022)

• Record Type: Journal Article
• Authors: Wang, Liqi; Zhang, Hairong; Li, Ziyue; Qu, Jiayao; Xing, Kaiwen; Wang, Minghao; Han, Cuiping; Qiu, Zeguo; Yu, Dianyu
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 170(2022)
• Publication Date: 2022-12-01
• Subjects: Photoelectrochemical biosensor -- Three-dimensional graphene oxide -- Lipase activity -- Detection; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2022.114076
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: In recent years, photoelectrochemical (PEC) biosensors have shown great application prospects in biological analysis and food detection. In this work, we developed and tested a new PEC biosensor. Three-dimensional graphene oxide (3DGO) was generated and deposited on an indium tin oxides (ITO) electrode. The photoelectrically active poly[Nile blue] (PNb) was formed on this electrode by electropolymerization, and glycerol dehydrogenase (GDH) was immobilised to generate the GDH-PNb-3DGO-ITO biosensor. This newly developed PEC biosensor exhibited excellent photoelectric response to lipase activity under light conditions, with a detection range of 0.1–6 U/mL and a lower detection limit of 0.069 U/mL. When the GDH-PNb-3DGO-ITO sensor was used for PEC detection of lipase activity by the standard addition recovery method, the recovery rate of the standard addition was 98.68–105.41%, and the relative standard deviation (RSD) was 2.1–3.5%, with good storage stability and repeatability. Therefore, this work provides new ideas for the design and preparation of PEC biosensors and introduces a new detection method for monitoring lipase activity. Graphical abstract: Image 1 Highlights: Development of a PEC biosensor to detect lipase activity. The fabricated biosensor has excellent optoelectronic properties. The sensor has a low detection limit (0.069 U/mL), providing a platform for lipase activity detection.