Microbiome-metabolomics analysis insight into the effects of dietary resistant starch on intestinal integrity. (1st February 2023)

• Record Type: Journal Article
• Authors: Qin, Simeng; Zhang, Keying; Ding, Xuemei; Bai, Shiping; Wang, Jianping; Tian, Gang; Xuan, Yue; Su, Zhuowei; Zeng, Quifeng
• Is Part Of: Food chemistry. Volume 401(2023)
• Publication Date: 2023-02-01
• Subjects: Resistant starch -- Gut integrity -- Microbiome -- Metabolomics -- Meat ducks; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134148
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Raw potato-resistant starch (RS) had better performance in enhancing gut integrity. RS contributed to growth of beneficial bacteria and butyrate-producing bacteria. Amino acid metabolism was typical metabolic pathway altered by RS administration. Metabolites altered by resistant starch had highly correlation with gut microbiota. Abstract: Resistant starch (RS) has caught much attention for its potential to exert a beneficial impact on intestine and certain members of its resident microbiota. In this study, we examined how dietary RS promotes intestinal barrier in meat ducks by microbiome-metabolomics analysis. Ducklings were fed corn-soybean basal diet or RS diet. Dietary RS improved intestinal morphology and enhanced barrier function in ileum, evidenced by lower permeability and upregulated tight junction proteins and Mucin-2 gene expression. Microbiome analysis showed that RS administration elevated the proportion of Firmicutes and butyrate-producing bacteria, and increased butyrate contents in cecum. Furthermore, significant alterations in metabolic profiles were observed, with most of these were associated with the amino acid metabolism (especially tryptophan), lipid metabolism, and intestinal inflammation. Together, diet with RS improved gut integrity and caused corresponding alterations in gut metabolome and microbiome, yielding better insights of the mechanism by RS improved the gut system of ducks.