Glycosylation reduces the allergenicity of turbot (Scophthalmus maximus) parvalbumin by regulating digestibility, cellular mediators release and Th1/Th2 immunobalance. (15th July 2022)

• Record Type: Journal Article
• Authors: Wu, Yeting; Lu, Youyou; Huang, Yuhao; Lin, Hong; Chen, Guanzhi; Chen, Yan; Li, Zhenxing
• Is Part Of: Food chemistry. Volume 382(2022)
• Publication Date: 2022-07-15
• Subjects: CD Circular dichroism -- ELISA Enzyme-linked immunosorbent assay -- SGF Simulated gastric fluid -- SIF Simulated intestinal fluid -- TPV native turbot parvalbumin -- H-TPV heated turbot parvalbumin -- G-TPV Glycated turbot parvalbumin -- OPA O-phthaldialdehyde assay; Allergenicity -- Turbot parvalbumin -- Glycosylation modification -- In vitro digestibility -- Cellular mediators -- Th1/Th2 immunobalance; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132574
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Glycosylation treatments of turbot PV( T -PV) resulted in crosslinks formation. Glycosylation caused changes in the SGF and SIF digestion stability of T -PV. Glycated T -PV exhibited a weaker allergic reaction than native T -PV. Glycosylation to T -PV reduced the release of cellular mediators IL-4/IL-13. Glycosylation to T -PV decreased allergic responses by downregulating Th2 cytokines. Abstract: With turbot being increasingly consumed, turbot parvalbumin (TPV) allergy has become a pressing problem requiring immediate resolution. Glycosylation treatment not only resulted in cross-link formation but also caused changes in the simulated gastric fluid and simulated intestinal fluid digestion stability of TPV. In addition, KU812 experimentation revealed lower levels of β-hexosaminidase, histamine, tryptase, interleukin 4 (IL-4)/IL-13 in glycated protein-treated mice compared with native PV–treated ones. Glycated TPV exhibited a weaker allergic reaction compared with native TPV. Systemic anaphylaxis resulted in mild anaphylactic responses and reduced temperature, along with significantly increased levels of immunoglobulin E and histamine. Furthermore, glycosylation treatment reduced the release of cellular mediators and cytokines (IL-4/IL-13). Glycation to T -PV decreased allergic responses by downregulating Th2 cytokines, regulated the Th1/Th2 balance and effectively reduce the allergenicity and sensitisation ability of T -PV.