Phenolics from noni (Morinda citrifolia L.) fruit alleviate obesity in high fat diet-fed mice via modulating the gut microbiota and mitigating intestinal damage. (15th February 2023)

• Record Type: Journal Article
• Authors: Wang, Ruimin; Wang, Lu; Wang, Shengwei; Wang, Juntao; Su, Congyan; Zhang, Lin; Li, Congfa; Liu, Sixin
• Is Part Of: Food chemistry. Volume 402(2023)
• Publication Date: 2023-02-15
• Subjects: Noni fruit -- Phenolics -- Gut microbiota -- Intestinal barrier function -- Intestinal inflammation; HFD high-fat diet -- SCFA short-chain fatty acid -- LPS lipopolysaccharide -- WHO World Health Organization -- NFE noni fruit phenolic extract -- 16S rRNA 16S ribosomal RNA -- HPLC high-performance liquid chromatography -- TG triglyceride -- TC total cholesterol -- LDL-C low-density lipoprotein cholesterol -- HDL-C high-density lipoprotein cholesterol -- MPO myeloperoxidase -- MDA malondialdehyde -- CAT catalase -- GSH glutathione -- ELISA Enzyme-linked immunosorbent assay -- TNF-α tumor necrosis factor-α -- IL-6 interleukin-6 -- IL-1β interleukin-1β -- ZO-1 zonula occludens-1 -- TLR4 toll-like receptor 4 -- MyD88 myeloid differentiation factor 88 -- NF-κB nuclear factor kappa-B -- DAD diode array detector -- ND normal chow diet group -- NFE normal chow diet with high-dose NFE group -- HFD high-fat diet group -- NFEL high-fat diet with low-dose NFE group -- NFEH high-fat diet with high-dose NFE group -- WAT white adipose tissue -- H&E hematoxylin and eosin -- FITC fluorescein isothiocyanate isomer -- DAPI 4′, 6-diamidino-2-phenylindole -- FID flame ionization detector -- DNA deoxyribonucleic acid -- OTUs operational taxonomic units -- RDP Ribosomal Database Project -- BCA Butyleyanoacrylate -- SDS-PAGE sulfate polyacrylamide gel electrophoresis -- PVDF poly(1, 1-difluoroethylene) -- SD standard deviation -- ANOVA analysis of variance -- PCA principal components analysis -- PCoA. principal co-ordinates analysis; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134232
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: NFE intervention decreased body weight and lipid accumulation in HFD-fed mice. NFE ameliorated HFD-induced gut dysbiosis. NFE increased SCFA-producing bacteria and decreased LPS-producing bacteria. NFE mitigated intestinal oxidative stress and barrier dysfunction. NFE blocked LPS/TLR4/NF-κB pathway to attenuate intestinal inflammation. Abstract: Noni fruit has certain anti-obesity effect. However, the bioactive ingredients in noni fruit that contribute to anti-obesity activity as well as the relation between its anti-obesity activity and gut microbiota remain unclear. In this study, obese mice induced by high-fat diet (HFD) and were intervened with noni fruit phenolic extract (NFE) for 10 weeks. The results showed NFE supplementation decreased body weight, lipid accumulation in liver and adipose tissues, ameliorated gut microbiota dysbiosis by increasing short-chain fatty acid (SCFA)-producing bacteria and decreasing lipopolysaccharide (LPS)-producing bacteria, and mitigated intestinal inflammation and oxidative stress. Moreover, NFE supplementation improved intestinal barrier dysfunction by elevating the protein expression levels of Claudin-1, Occludin and ZO-1, alleviated the HFD-induced intestinal inflammation by repressing the LPS/TLR4/NF-κB pathway. Collectively, the findings revealed NFE intervention inhibits obesity by improving gut microbiota disorder, barrier function, and inflammation. Hence, NFE may be an effective way to ameliorate HFD-induced damage.