Glycoproteomics reveal differences in site-specific N-glycosylation of whey proteins between donkey colostrum and mature milk. (1st November 2022)

• Record Type: Journal Article
• Authors: Guan, Boyuan; Gao, Yang; Chai, Yuxia; Xiakouna, Amantai; Chen, Xinping; Cao, Xueyan; Yue, Xiqing
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 169(2022)
• Publication Date: 2022-11-01
• Subjects: Donkey milk -- Colostrum -- Mature milk -- Whey N-Glycoprotein -- Site-specific glycoproteomics; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2022.113920
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Donkey whey protein has high nutritional value, yet systematic characterisation of site-specific N-glycosylation of donkey whey protein is still in its infancy. This study used label-free site-specific glycoproteomics to identify and quantify 685 intact N-glycopeptides from 135 whey glycoproteins in donkey colostrum (DC) and 384 intact N-glycopeptides from 67 glycoproteins in donkey mature milk (DM). As lactation progressed, 23 site-specific N-glycans mapping to 13 glycosites on 12 glycoproteins were significantly decreased, and 19 site-specific N-glycans mapping to 7 glycosites on 6 glycoproteins were significantly increased. We also found that certain N-glycans attached to the glycosites exhibited significant site specificity. This study provides new insights into the potential relationship between the biological function of donkey whey proteins and their N-glycosylation modifications. Furthermore, the study provides molecular evidence for differences in the site-specific N-glycosylation profile of donkey whey protein during lactation. Graphical abstract: Image 1 Highlights: Donkey whey protein site-specific N-glycosylation differs during lactation. 946 N-glycopeptides were identified in 164 donkey whey proteins during lactation. Heterogeneity of N-glycosylation of donkey whey proteins is outlined. 42 N-glycopeptides of 14 donkey whey proteins differ significantly during lactation. Structure-activity relationship of donkey whey N-glycoproteins is elucidated.