Ethanolic extract of Coleus aromaticus leaves impedes the proliferation and instigates apoptotic cell death in liver cancer HepG2 cells through repressing JAK/STAT cascade. Issue 10 (9th August 2022)

• Record Type: Journal Article
• Authors: Shekh, Rafia; Tiwari, Rohit Kumar; Ahmad, Afza; Ahmad, Irfan; Alabdallah, Nadiyah M.; Saeed, Mohd; Ansari, Irfan Ahmad; Mishra, Anuradha; Ashfaque, Mohammad; Bajpai, Preeti
• Is Part Of: Journal of food biochemistry. Volume 46:Issue 10(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-08-09
• Subjects: anticancer -- apoptosis -- Coleus aromaticus -- HepG2 cells -- liver cancer; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Biochemistry -- Periodicals; 664.024
• DOI: 10.1111/jfbc.14368
• Languages: English
• ISSNs: 0145-8884

Abstract

Abstract: Liver cancer or hepatocellular carcinoma (HCC) has become a leading cause for cancer burden across the globe, and incidences have tripled since the last two decades. Poor diagnosis of primary liver cancer and limited treatment strategies aggravate the challenges. Researchers globally have shown a steep inclination toward the exploration of plant‐based compounds for their nutraceutical and anticancer potential to fit into the role of novel chemotherapeutics. Coleus aromaticus is a well‐known culinary herb that earlier has been reported for several medicinal attributes. The current investigation deals with exploring the anticancer potential of ethanolic leaf extract of C. aromaticus ( Co L‐EtOH) against hepatocellular carcinoma HepG2 cell line. The observations made it evident that Co L‐EtOH extract impeded the viability of HepG2 at 400 μg/ml ( p < .01). Additionally, the extract also succeeded in escalating ROS production ( p < .01) which aided dissipation of mitochondrial membrane potential and disruption of nuclear morphology. Co L ‐EtOH further activated caspase‐8, −9, and −3 which was reaffirmed by increase in apoptosis at 400 μg/ml ( p < .01). Moreover, post treatment with Ca LEt‐OH extract significantly reduced the expression of JAK‐1 & STAT‐3 genes ( p < .01) along with regulated expression of Mcl1, Bcl‐2, cyclinD1, p21, and p27 within HepG2 cells. This evidence portrays the promising anticancer potential of Co L‐EtOH projecting it as a novel chemotherapeutic agent against HCC. Practical applications: The herb Coleus aromaticus belonging to Lamiaceae family and Coleus genus is known by various names in different regions of the world and several language‐specific vernacular names. The herb has been used in therapeutic and medicinal applications as well as in culinary preparations. Various attributes of the nutritional strength and functional characteristics of the leaves in terms of carotenoids, minerals, phenols, dietary fiber, and antioxidant activity have been reported by several researchers. Carvacrol and thymol are majorly found in the plant, while chlorogenic acid and rosmarinic acid etc. as the phenolic components. The herb has been used in therapeutic and medicinal implications as well as in culinary preparations. Abstract : Ethanolic leaf extract of Coleus aromaticus instigated apoptotic cell death in human lung cancer A549 cells. The antiproliferative effect of C. aromaticus extract was found to be mediated by impeded JAK/STAT signaling pathway. The findings strongly potentiate the promising anticancer potential of C. aromaticus leaves ethanolic extract against hepatocellular carcinoma.