Rapid identification of escolar (Lepidocybium flavobrunneum) and oilfish (Ruvettus pretiosus) species based on recombinase polymerase amplification. (February 2023)

• Record Type: Journal Article
• Authors: Xie, Ruibin; Zhang, Juan; Wen, Shuang; Zhao, Ruiming; Wang, Nan; Chen, Ailiang
• Is Part Of: Food control. Volume 144(2023)
• Publication Date: 2023-02
• Subjects: Escolar -- Oilfish -- Isothermal amplification -- Rapid detection -- Recombinase polymerase amplification; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2022.109352
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Escolar or oilfish being sold as cod is a worldwide concerning food counterfeiting issue. This study established a simple and rapid real-time recombinase polymerase amplification (RPA) assay for oilfish ( Ruvettus pretiosus ) and escolar ( Lepidocybium flavobrunneum ) detection. The assay uses RPA primers and a probe designed for the intraspecific conserved and interspecific regions in the COI and Cyt b genes, respectively. The RPA assay was completed within 20 min at 37 °C, was specific for either oilfish or escolar species, and showed no cross-reactivity with other cod or salmon fishes. The limit of detection for oilfish and escolar was 9.6 pg/μL and 48 pg/μL, respectively. Samples that were cooked or digested retained a detection sensitivity of 1%. This real-time RPA assay is a simple, rapid, reliable, and sensitive method for oilfish or escolar identification which may help control oilfish or escolar adulteration and ensure consumer health. Graphical abstract: Image 1