In Vitro Infant Fecal Fermentation Characteristics of Human Milk Oligosaccharides Were Controlled by Initial Microbiota Composition More than Chemical Structure. Issue 19 (31st August 2022)

• Record Type: Journal Article
• Authors: Xu, Shiqi; Lane, Jonathan A.; Chen, Juchun; Zheng, Yuxing; Wang, Hongwei; Fu, Xiong; Huang, Qiang; Dhital, Sushil; Liu, Feitong; Zhang, Bin
• Is Part Of: Molecular nutrition & food research. Volume 66:Issue 19(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-08-31
• Subjects: human milk oligosaccharides -- in vitro fecal fermentation -- infant gut microbiota -- metabolites -- molecular specificity; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.202200098
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: Human milk oligosaccharides (HMOs), multifunctional glycans naturally present in human milk, are known to contribute to the infant's microbiota and immune system development. However, the molecular specificity of HMOs on microbiota and associated fermentation is not yet fully understood, and is important for the development of infant formula optimum functionality. Methods and results: In vitro fermentation is carried out on structurally different HMOs with infant fecal inocula dominated by Bifidobacterium longum, Bifidobacterium breve, and Bacteroides . The gas, metabolite (SCFA, lactate, and succinate) profiles, and microbiota responses differ between individual microbiota inocula patterns regardless of HMO structure. In terms of HMO pairs with same sugar composition but different glycosidic bonds, gas and metabolite profiles are similar with the B. longum‐ and B. breve ‐dominated inocula. However, large individual variations are observed with the Bacteroides ‐dominated inocula. The microbial communities at the end of fermentation are closely related to the initial microbiota composition. Conclusion: The findings demonstrate that short‐term in vitro fermentation outcomes largely depend on the initial gut microbiota composition more than the impact of HMO molecular specificity. These results advance the current understanding for the design of personalized infant nutritional solutions and therapies in future. Abstract : Human milk oligosaccharides (HMOs) are beneficial to infant health. An in vitro fermentation trial with individual Bifidobacterium longum‐, Bifidobacterium breve‐, and Bacteroides ‐dominated infant fecal inocula is performed. This study demonstrates the HMO fermentation profiles are more dependent on the initial gut microbiota composition than chemical structure of HMOs. These preliminary findings shed new light on designing personalized infant formulas and relevant therapies.