A phage-based magnetic relaxation switching biosensor using bioorthogonal reaction signal amplification for Salmonella detection in foods. (30th January 2023)

• Record Type: Journal Article
• Authors: Huang, Chenxi; Zhao, Junpeng; Lu, Rongsheng; Wang, Jia; Nugen, Sam R.; Chen, Yiping; Wang, Xiaohong
• Is Part Of: Food chemistry. Volume 400(2023)
• Publication Date: 2023-01-30
• Subjects: Phage -- Magnetic relaxation switching (MRS) -- Bioorthogonal reaction -- Signal amplification -- Salmonella detection; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134035
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A novel phage-based MRS sensor was proposed for Salmonella detection. Bioorthogonal reaction TCO/Tz improves the sensitivity of this sensor. This sensor has a LOD of 5 CFU per mL with a linear range of 10 2 -10 8 CFU per mL. The detection limit was below 10 CFU/25 mL with 4-h pre-enrichment. Abstract: Phages are uniquely suited for bacterial detection due to their low cost and ability to recognize live bacteria. Herein, our work establishes the proof-of-concept detection of Salmonella in orange juice based on a phage-mediated portable magnetic relaxation switching (MRS) biosensor. The limit of quantification (LOQ) could reach 5 CFU/mL (95 % confidence interval [CI]: 4–7, N = 4) with a linear range of 10 2 -10 8 CFU/mL, which has improved 10-fold than that without bioorthogonal signal amplification. The recovery rate of the phage-based MRS biosensor was 95.0 % (95 % confidence interval [CI]: 89.0 %-100.9 %, N = 6). The specificity of the phage-based MRS biosensor was 100 % without false-positive results. In addition, this sensor was able to detect <10 CFU per 25 mL of Salmonella in orange juice with 4-h pre-enrichment. The result from the phage-based MRS biosensor is consistent with that from the standard plate count method. This sensor provides a reliable and ultrasensitive detection platform for pathogens.