Taking glucose as intermediate bridge-signal-molecule for on-site and convenient detection of ochratoxin A in rice with portable glucose meter. (30th January 2023)

• Record Type: Journal Article
• Authors: Zhang, Ru; Yan, Chao; Zong, Ziwen; Qu, Wei; Yao, Li; Xu, Jianguo; Zhu, Yingyue; Yao, Bangben; Chen, Wei
• Is Part Of: Food chemistry. Volume 400(2023)
• Publication Date: 2023-01-30
• Subjects: Ochratoxin A -- Electrochemical aptasensor -- Gold nanoparticles -- Personal glucose meter -- Food safety; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134007
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Rapid ochratoxin A (OTA) detection with the commercial portable glucose meter. Glucose as the intermediate bridge molecule for OTA detection. Aptamer based recognition system integrated with the invertase catalysis system. OTA sensing event transferred to catalytic glucose production system. OTA detection results can be directly judged from portable glucose meter. Abstract: On-site screening of biotoxins is of great importance for food safety. A new electrochemical-biosensing strategy was constructed for ochratoxin A (OTA) detection by direct using ready-made commercial portable-glucose-meter (PGM). Aptamer against OTA was adopted as the recognition probe and pre-immobilized onto the sensing interface. The complementary biotin-modified probe was further decorated by hybridization. Biotinylated invertase was further introduced onto the sensing system with streptavidin, which also acted as the signal amplification unit. The invertase, which was depended on the amount of OTA, produced the glucose from sucrose in the sensing solution. The glucose could be directly and conveniently measured with PGM. Quantitative analysis of OTA was achieved with a linear range from 0.5 ng/mL to 10 ng/mL and detection limit of 0.45 ng/mL. Of significance, it has been successfully applied for OTA analysis in rice with satisfied recoveries. This unique PGM-based electrochemical platform reveals prospective potential in food safety monitoring.