Ultrasonic treatment of Dendrobium officinale polysaccharide enhances antioxidant and anti‐inflammatory activity in a mouse D‐galactose‐induced aging model. Issue 8 (1st April 2022)

• Record Type: Journal Article
• Authors: Chu, Wenhui; Wang, Pan; Ma, Zhe; Peng, Lin; Wang, Zongmin; Chen, Zilin
• Is Part Of: Food science & nutrition. Volume 10:Issue 8(2022)
• Page Start: 2620
• Page End: 2630
• Publication Date: 2022-04-01
• Subjects: anti‐inflammatory activity -- antioxidant activity -- Dendrobium officinale -- D‐galactose -- polysaccharide; Food industry and trade -- Periodicals; Food -- Periodicals; Nutrition -- Periodicals; 664
• DOI: 10.1002/fsn3.2867
• Languages: English
• ISSNs: 2048-7177

Abstract

Abstract: Utilization of the biological macromolecule Dendrobium officinale polysaccharide (DOP) as a functional ingredient is limited by its high intrinsic viscosity and molecular weight. The goal of the present study was to improve rheological properties of DOP by ultrasonic treatment. Such a treatment resulted in the degradation of DOP and consequent reduction of rheological properties. Among DOP samples treated with ultrasonication at low (L), medium (M), and high (H) power intensities (25, 50, 75 w/cm 2 ), M‐DOP displayed the highest reactive oxygen species (ROS) and reactive nitrogen species (RNS) radical scavenging activity in vitro. In a mouse D‐galactose (D‐Gal)‐induced aging model, M‐DOP significantly increased activities of antioxidant enzymes and reduced levels of pro‐inflammatory cytokines in liver. Real‐time polymerase chain reaction (RT‐PCR) analysis indicated that M‐DOP upregulated messenger RNA (mRNA) expression of anti‐inflammatory/antioxidant proteins such as Nrf2 (nuclear factor erythroid 2‐related factor), hemeoxygenase‐1 (HO‐1), and NAD(P)H:quinone oxidoreductase (NQO1) in liver. In summary, M‐DOP displayed a strong radical scavenging activity in vitro, and ameliorated liver injury in the mouse aging model through the promotion of Nrf2/HO‐1/NQO1 signaling pathway. Abstract : After the ultrasonic treatment, the rheological property and structural properties of Dendrobium officinale polysaccharide were improved. Ultrasonic treatment also improved the antioxidant and antiaging activity in D‐galactose (D‐Gal) mice. Treated polysaccharide reduced liver injury in D‐galactose mice through the promotion of Nrf2/HO‐1/NQO1 (nuclear factor erythroid 2‐related factor/hemeoxygenase‐1/NAD(P)H:quinone oxidoreductase) signaling pathway.