Fluorescence-based analyser as a rapid tool for determining soluble protein content in dairy ingredients and infant milk formula. (March 2019)

• Record Type: Journal Article
• Authors: Henihan, Lisa E.; O'Donnell, Colm P.; Esquerre, Carlos; Murphy, Eoin G.; O'Callaghan, Donal J.
• Is Part Of: Innovative food science & emerging technologies. Volume 52(2019)
• Page Start: 75
• Page End: 79
• Publication Date: 2019-03
• Subjects: Abbreviation description -- agg agglomerated powder -- CIF commercial infant milk formula -- k number of measurements -- MIF model infant milk formula -- min minutes -- n number of samples -- NCN non-casein nitrogen -- NPN non-protein nitrogen -- RP-HPLC reverse phase high performance liquid chromatography -- RSD relative standard deviation, SD/mean × 100 -- SD standard deviation -- SDS-PAGE SDS-polyacrylamide gel electrophoresis -- SEest standard error of the estimate, measure of the variability of predictions in a regression -- SMP skim milk powder -- SP soluble whey protein content g/100 g -- SPKjeldahl soluble whey protein content by Kjeldahl -- std non agglomerated powder -- TN total nitrogen -- TS % total solids -- WPC whey protein concentrate -- WPN undenatured whey protein nitrogen, mg per g of non-fat milk powder -- WPNI whey protein nitrogen index -- WPNIF whey protein nitrogen index by fluorescence based analyser -- WPNIGEA whey protein nitrogen index by GEA Niro method; Dairy ingredients -- Infant milk formula -- Fluorescence-based analyser -- Whey protein nitrogen index -- Soluble protein -- Process analytical technology; Food -- Biotechnology -- Periodicals; Food industry and trade -- Technological innovations -- Periodicals; Aliments -- Biotechnologie -- Périodiques; Food -- Biotechnology; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.ifset.2018.11.014
• Languages: English
• ISSNs: 1466-8564

Abstract

Abstract: Milk protein, in particular native whey protein, is of interest to dairy manufacturers as a measure of functional and nutritional quality. However, quantification of soluble whey protein (SP) is time consuming; giving rise to the need to develop rapid, accurate, and portable at-line process analytical technology. In this study, the performance of a fluorescence-based analyser(F ) (Amaltheys II, Spectralys Innovations, France) was evaluated for quantification of SPF and whey protein nitrogen index (WPNI)F in skim milk, whey protein concentrate and infant formula powders. Rehydration of powders prior to analysis was a key factor for ensuring repeatability and reproducibility. A comparison of the analyser with reference methods for SPF and WPNIF resulted in coefficient of determination (R 2 ) > 0.993 for both SPKjeldahl method and WPNIGEA . The results show the fluorescence-based analyser to be rapid, compact, and accurate device, suited for providing reliable support to dairy ingredient and infant formula manufacturers. Industrial relevance: The fluorescence based analysis investigated in this article is suitable for application in the dairy industry where it can be used as a rapid, at-line PAT tool for both liquid and powder samples. The technology has the potential to replace well-established methods for measurement of soluble protein. The main benefit to industry is the ability to respond more rapidly to variations in soluble protein without compromising on the accuracy associated with more time consuming methods. Highlights: Rapid and accurate at-line process analytical technology for dairy industry Fluorescence-based analyser for replacement of standard analytical methods Quantitative measurement of soluble whey protein and whey protein nitrogen index