Simulated digestion and fermentation in vitro with human gut microbiota of polysaccharides from Coralline pilulifera. (February 2019)

• Record Type: Journal Article
• Authors: Wang, Yidan; Chen, Guijie; Peng, Yujia; Rui, Ying; Zeng, Xiaoxiong; Ye, Hong
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 100(2019)
• Page Start: 167
• Page End: 174
• Publication Date: 2019-02
• Subjects: Coralline pilulifera polysaccharides -- Polysaccharides digestion -- Polysaccharides fermentation -- Gut microbiota -- Prebiotics polysaccharides; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2018.10.028
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Crude polysaccharides from Coralline pilulifera (CPPS) were purified by DEAE Sepharose Fast Flow to afford four purified fractions of CPPS-1, CPPS-2, CPPS-3 and CPPS-4. The physicochemical property and digestion under simulated saliva, gastric, small intestinal conditions and fermentation of CPPS-3 were investigated in vitro . CPPS-3 was composed of glucose and galactose, its molecular weight was 716 kDa, the contents of carbohydrate, protein, uronic acid and sulfuric radical were 67.84%, 1.00%, 0.38% and 9.07%, respectively. There was no significant difference on the molecular weight and content of reducing sugars, and no monosaccharides were released after saliva, gastric and small intestine digestion, indicating that CPPS-3 could not be digested under the simulated digestion conditions. After fermentation for 24 h in vitro, the carbohydrate content was decreased to 30.76%, suggesting that CPPS-3 could be broken down and utilized by gut microbiota. Some health-promoting gut microbiota such as Bacteroides, Megamonas and Veillonella were significantly enhanced, suggesting that CPPS-3 could regulate the composition of gut microbiota. What's more, CPPS-3 could promote the productions of short-chain fatty acids (22.17 and 16.17 mmol/L at 24 h for CPPS-3 and control, respectively), which could contribute to host health. Therefore, CPPS-3 is expected to be developed as functional foods. Graphical abstract: Image 1 Highlights: Fraction 3 of Coralline pilulifera polysaccharides was sulfated polysaccharide. CPPS-3 was not digested under simulated saliva, gastric and intestinal juices. CPPS-3 reached large intestine safely and was utilized by intestinal microbiota. CPPS-3 could regulate the structure of gut microbiota in vitro. CPPS-3 significantly promoted the secretion of SCFAs such as acetate, propionate.