Simultaneous determination of multi-pesticide residues in fish and shrimp using dispersive-solid phase extraction with liquid chromatography–tandem mass spectrometry. (February 2021)

• Record Type: Journal Article
• Authors: Shin, Dasom; Kim, Joohye; Kang, Hui-Seung
• Is Part Of: Food control. Volume 120(2021)
• Publication Date: 2021-02
• Subjects: Pesticide -- Multi-residue -- Aquatic animal -- d-SPE -- LC–MS/MS -- Analytical method; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2020.107552
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: In this work, we developed and optimized an analytical method for the simultaneous determination of multi-pesticide residues in fishery products using dispersive-solid phase extraction and liquid chromatography–tandem mass spectrometry (LC–MS/MS). We selected 66 pesticides (insecticide, fungicides, and herbicides) and determined their residue concentrations in fish and shrimp. The samples were prepared according to the modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure. Matrix-matched calibrations were carried out at three different spiking levels in flatfish, eel, and shrimp. The validation procedure was performed to determine the accuracy, precision, limits of detection, and limit of quantification (LOQ) according to the Codex guidelines (CAC/GL-71). Consequently, the proposed method is satisfactory in terms of accuracy (recoveries between 70 and 125%), precision (relative standard deviation below 20%). The matrix effects were between −98.1 and 48.6% in flatfish, eel, and shrimp. LOQs of this method was below 10 μg kg −1 . The proposed method was applied to investigate the pesticide residue levels of fresh fish and shrimp samples ( n = 78) obtained from domestic markets in Korea. As a result, 10 samples was positive (12.8%) for the 66 targeted pesticides. Based on our results, the developed method was successfully applied for the detection and quantification of pesticide residues in fish and shrimp. Highlights: We proposed multi-residue analytical method for determination of pesticide residues. The method showed satisfactory validation performance according to CODEX guideline. The simplicity, high throughput, reliability of results were the advantage of method. The evaluated matrix effects (−98.1 and 48.6%) varied with species (flatfish, eel, and shrimp). Among 78 analyzed samples, 12.8% ( n = 10) was detected in fish and shrimp.