Green extraction of bioactive compounds from apple pomace by ultrasound assisted natural deep eutectic solvent extraction: Optimisation, comparison and bioactivity. (1st January 2023)

• Record Type: Journal Article
• Authors: Rashid, Rubiya; Mohd Wani, Sajad; Manzoor, Shaziya; Masoodi, F.A.; Masarat Dar, Mohd
• Is Part Of: Food chemistry. Volume 398(2023)
• Publication Date: 2023-01-01
• Subjects: Deep eutectic solvent -- Apple pomace -- Bioactive compound -- Ultrasound assisted extraction; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.133871
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Apple pomace possesses good amount of phenolic and flavonoid compounds. Deep eutectic solvents presented greener option for extraction of bioactive compounds. UAE coupled with deep eutectic solvent exhibits higher extraction efficiency. Optimised UAE enhances the extraction of polyphenols from apple pomace. Abstract: Natural deep eutectic solvents (NADES) coupled with ultrasound assisted extraction (UAE) was evaluated as extraction techniques for bioactive compounds from apple pomace. Seven natural deep eutectic solvents (NADES) with different molar ratios were explored as green extraction solvents to evaluate extraction efficiency in terms of total flavonoid content (TFC), total phenolic content (TPC), lipid peroxidation inhibition (LBI) and antioxidant potential. The solvents which performed better than conventional solvent were DES1 (Choline chloride: glycerol (1:2)), DES2 (Choline chloride: lactic acid (1:3)) and DES5 ((Choline chloride: citric acid (1:1)). These solvents were further evaluated for optimisation of processing parameters in UAE-DES. The maximum TPC value was achieved using sonication time 40 min, water 30 %, solid/liquid ratio 1:30, temperature 40 °C, acoustic intensity 83.2 W/cm 2 and duty cycle 75 %. HPLC of optimised extract was utilised for quantification of polyphenol compounds showing quercetin as main compound followed by chlorogenic acid, gallic acid, phloretin, phloridizin and rutin.