Improving the stability and bioactivity of curcumin using chitosan-coated liposomes through a combination mode of high-pressure processing. (1st October 2022)

• Record Type: Journal Article
• Authors: Chen, Wei-Ting; Kuo, Yu-Lin; Chen, Chien-Hua; Wu, Hung-Ta; Chen, Hua-Wei; Fang, Wu-Po
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 168(2022)
• Publication Date: 2022-10-01
• Subjects: Chitosan -- Liposomes -- Curcumin -- High-pressure processing -- Thermal degradation; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2022.113946
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: This study used chitosan-coated liposomes of curcumin (cur-chitosomes) coupled with a combination mode of high-pressure processing (HPP) and ethanol injection to improve the bioactivity, preservation, and thermal stability of curcumin. The results revealed cur-chitosomes presented the best thermal stability and encapsulation efficiency. With 0.1% chitosan addition, the encapsulation efficiency increased from 73.8 to 84.0%, accompanying with less increases on particle size and polydispersity index. Storage stability for 28 days incubation demonstrated the particle size of cur-chitosomes remained at a constant value, which presented the long thermal stability due to the static electrostatic repulsive force. The stability of cur-chitosomes presented high value of 85.3% at degradation conditions of 25 °C and 480 min. With thermal kinetic calculation, the half-life of curcumin-loaded liposomes and cur-chitosomes were 625 and 1250 min at 25 °C, so liposome formation and chitosan addition in curcumin could increase the storage time. Over 10 4 CFU/g Escherichia coli, Staphylococcus aureus, and Staphylococcus aureus could be completely sterilized in the preparation of cur-chitosomes at 400 MPa HPP. Consequently, chitosomes by HPP not only prevented the thermal degradation of heat-sensitive substances but also enhanced the storage stability and the sterilization effect in a non-thermal process. Graphical abstract: Image 1 Highlights: HPP has been innovatively used to produce chitosan-coated liposomes of drug. Formation of cur-chitosomes by the HPP was the fast-adiabatic expansion of water. Cur-chitosome by HPP exhibited enhanced stability compared with probe-sonication. HPP as a stabilizing agent can avoid thermal degradation of bioactive compounds. HPP is simultaneously applied to produced liposomes and inactivate microorganisms.