Phytochemical composition, biological propensities, and in-silico studies of Crateva adansonii DC.: A natural source of bioactive compounds. (October 2022)

• Record Type: Journal Article
• Authors: Pervaiz, Irfan; Hasnat, Muhammad; Ahmad, Saeed; Khurshid, Umair; Saleem, Hammad; Alshammari, Farhan; Almansour, Khaled; Mollica, Adriano; Rengasamy, Kannan R.R.; Zainal Abidin, Syafiq Asnawi; Anwar, Sirajudheen
• Is Part Of: Food bioscience. Volume 49(2022)
• Publication Date: 2022-10
• Subjects: Crateva adansonii -- phytochemicals -- Antioxidant -- Enzyme inhibition -- Docking; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2022.101890
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: The current research work aimed at the phytochemical, biological, and in-silico properties of hydro-methanol extract and n -hexane fraction of Crateva adansonii aerial parts. Total bioactive content and UHPLC-MS analysis were used to determine the phytochemical composition. Regarding biological activities, antioxidant (DPPH, ABTS, FRAP, CUPRAC, phosphomolybdenum, and metal chelation) and enzyme inhibition (against α-amylase, tyrosinase, acetylcholinesterase -AChE, and butyrylcholinesterase -BChE) activities were assessed. The hydro-methanol extract contained higher phenolic (28.09 mg GAE/g extract) and flavonoid (65.61 mg RE/g extract) contents, which might be correlated with the higher antioxidant potential of this extract. For the enzyme assays, the n -hexane fraction was most active against BChE and α-amylase, while the methanol extract presented higher inhibition values against AChE and tyrosinase enzymes. Likewise, different phytocompounds have been tentatively identified by UHPLC-MS of hydro-methanol extract; most of these belonged to phenolic and flavonoid classes. Furthermore, three important identified phytocompounds (isovitexin, robinetin 3-rutinoside, and luteolin 7-robinobioside), were selected for in-silico computational studies against the tyrosinase enzyme. The present investigation revealed that the studied extract/fraction of C. adansonii are likely sources of phytoconstituents identified through UHPLC-MS with considerable antioxidant and enzyme inhibitory properties. To conclude, the evaluated plant should be further investigated for the isolation of bioactive molecules which can be utilized in phytopharmaceuticals. Highlights: Crateva adansonii is a medicinal plant known to possess different traditional uses. Hydro-methanol and n -hexane solvent extract/fraction were screened for phytochemical, antioxidant, and enzyme inhibition activities. The hydro-methanol extract was found to contain considerable bioactive compounds and biological potential. The molecular docking studies further highlighted the interaction between the important phytochemicals and the tyrosinase enzymes.