Cellulose nanofibril-stabilized Pickering emulsion as a high-performance interfacial biocatalysis system for the synthesis of phosphatidylserine. (15th January 2023)

• Record Type: Journal Article
• Authors: Yin, Chengmei; Zhang, Haiyang; Mao, Xiangzhao
• Is Part Of: Food chemistry. Volume 399(2023)
• Publication Date: 2023-01-15
• Subjects: Cellulose nanofibril -- Pickering emulsion -- Interface catalysis -- Transphosphatidylation activity -- Cellulose-binding domain; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.133865
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: A simple ball-milling method with the aid of ionic liquid was successfully applied to product cellulose nanofibrils. A one-step immobilizing procedure was developed to produce the immobilized-on-cellulose enzyme. The CNF-CBD1 was used as Pickering emulsions stabilizers for efficient synthesis of phosphatidylserine. The Pickering emulsion obtained was an attractive Pickering interfacial biocatalysis platform. Abstract: An efficient and attractive platform for synthesizing phosphatidylserine is indispensable for driving large-scale industrial applications. Given the interfacial effect of phospholipase D (PLD), an adequate oil-water interface surface is essential for the catalytic efficiency of PLD. Herein, a simple ball-milling method with ionic liquid was successfully applied to produce cellulose nanofibrils and immobilized PLD with the cellulose-binding domain through a one-step procedure. Each droplet in the Pickering reaction system was regarded as a microreactor to provide sufficient oil-water interface to the active sites of PLD. The constructed Pickering emulsion system exhibited outstanding catalytic activity, achieving 95.4 % conversion after 2 h with the minimal amount of catalyst (0.8 wt% of CNF-CBD1 addition amount), which was 6-fold higher than the previously reported immobilized MCC-CBD1 and 21-fold higher than free PLD-CBD1. These results demonstrated a sustainable and high-performance biocatalysis method, paving the way for the efficient synthesis of novel phospholipids.