Identifying an emergent adulterant hydrochlorothiazide in food: A simple lateral flow strip with high sensitivity by time-resolved fluorescence. (January 2023)

• Record Type: Journal Article
• Authors: Shen, Runlin; Guan, Tian; Li, Zhaodong; Hong, Ziling; Dzantiev, Boris B.; Zherdev, Anatoly V.; Koidis, Anastasios; Yao, Xiaojun; Lei, Hongtao
• Is Part Of: Food control. Volume 143(2023)
• Publication Date: 2023-01
• Subjects: Hydrochlorothiazide -- Antibody -- Immunochromatography -- Strip -- Adulterant -- Time-resolved fluorescence -- Functional food; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2022.109265
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Hydrochlorothiazide, an antihypertensive and diuretic drug only for clinic purpose, was recently found as an adulterant in functional foods. Due to the potential health risk, it is urgent to develop a simple and rapid assay for its identification. In this work, two hapten structures were rationally designed based on molecular modeling, and a highly sensitive antibody to hydrochlorothiazide was obtained with 50% inhibitive concentration (IC50 ) of 6.7 ng/mL for the first time. A lateral flow strip assay was then developed based on time resolved fluorescence, demonstrating a cut-off value of 250 μg/kg to hydrochlorothiazide in functional capsules and tablets, and a negligible cross-reactivity to related compounds. The strip performance for real samples collected from the retail markets was consistent of that of confirmed method (LC-MS/MS). As an ideal tool, the proposed strip could be applied to identify adulterated hydrochlorothiazide in functional foods. Graphical abstract: Image 1 Highlights: Two novel hydrochlorothiazide haptens were rationally designed assisted by computer molecular modeling. A novel antibody against hydrochlorothiazide was produced with high specificity and sensitivity. A easy-use lateral flow strip was developed for identifying emergent adulterant hydrochlorothiazide in foods.