Cronobacter sakazakii CICC 21544 responds to the combination of carvacrol and citral by regulating proton motive force. (March 2020)

• Record Type: Journal Article
• Authors: Cao, Yifang; Zhou, Ailian; Zhou, Donggen; Xiao, Xinglong; Yu, Yigang; Li, Xiaofeng
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 122(2020)
• Publication Date: 2020-03
• Subjects: Cronobacter sakazakii -- Stress response mechanism -- Transcriptome sequencing -- Citral -- Carvacrol; Carvacrol -- PubChem CID: 10364 -- Citral -- PubChem CID: 638011 -- Ethanol -- PubChem CID: 702 -- SYBR GERRN Ⅰ -- PubChem CID: 56841760; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2020.109040
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Response mechanism of Cronobacter sakazakii under stress of combination of citral and carvacrol was investigated by transcriptome sequencing. Twenty-five significantly differentially expressed genes were identified to be closely related to the ability of C. sakazakii tolerating the stress after analysis, mainly involving glycerol metabolism; citrate metabolism; formate metabolism; ribosome function and transmembrane transport system. Combination of carvacrol and citral could cause dissipation of proton motive force (PMF) and the psp regulon is an important switch in the response of C. sakazakii to this condition. To maintain PMF, psp regulated cellular processes to reduce the consumption of cellular energy for cell membrane repair and proton consumption. In T1 group (low concentration group), C. sakazakii remained aerobic metabolism and expression of glycerol metabolism related genes were upregulated for cell membrane repair and proton consumption. In T2 group (high concentration group), bacteria convert from aerobic metabolism to anaerobic metabolism to reduce energy consumption and expression of genes in formate metabolism were upregulated to consume excessive intracellular protons; citrate lyase-related genes were upregulated to decompose citrate, the intermediate of the tricarboxylic acid cycle, to generate acetyl CoA for production of cell membrane phospholipids. Highlights: Citral and carvacrol caused dissipation of proton motive force of C. sakazakii. Psp regulon regulated cellular processes to maintain PMF. Energy preservation, membrane repair and proton consuming helped to maintain PMF.