Analysis of organophosphorus and pyrethroid pesticides in organic and conventional vegetables using QuEChERS combined with dispersive liquid-liquid microextraction based on the solidification of floating organic droplet. (30th March 2020)

• Record Type: Journal Article
• Authors: Mao, Xuejin; Wan, Yiqun; Li, Zhanming; Chen, Lin; Lew, Hueylee; Yang, Hongshun
• Is Part Of: Food chemistry. Volume 309(2020)
• Publication Date: 2020-03-30
• Subjects: QuEChERS quick, easy, cheap, effective, rugged and safe -- DLLME–SFO dispersive liquid–liquid microextraction based on solidification of a floating organic droplet -- OP organophosphorus -- PYR pyrethroid -- MRLs maximum residue limits -- d-SPE dispersive solid phase extraction -- DLLME dispersive liquid–liquid microextraction -- GC–MS gas chromatography-mass spectrometry -- HPLC high performance liquid chromatography -- PSA primary secondary amine -- EI electron impact -- SIM selected ion monitoring mode -- LODs limits of detection -- LOQs limits of quantification -- ANOVA analysis of variance -- LSD least significant difference -- OAD orthogonal array experimental design -- RSDs relative standard deviations; Pesticide -- Organic vegetable -- Dispersive liquid–liquid microextraction -- Gas chromatography-mass spectrometry -- Chemical hazard -- Food safety; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.125755
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A method to detect two categories of pesticides in vegetables was developed. An orthogonal array experimental design optimized the experiment condition. QuEChERs with DLLME-SFO provided good recovery of the pesticides. The method is more environmentally-friendly than traditional methods. The method was applied successfully to analyze pesticide residues in real samples. Abstract: A simple, sensitive and environmentally-friendly method for determining organophosphorus and pyrethroid pesticides in vegetables was developed to better evaluate the risk of consuming them. The pesticides in vegetables were extracted, purified and concentrated by using the QuEChERS (quick, easy, cheap, effective, rugged and safe method) combined DLLME-SFO (dispersive liquid–liquid microextraction based on solidification of floating organic droplet) techniques. The key parameters were optimized through orthogonal array experimental design and statistical analysis. The linearity of the calibration curves was satisfied in matrix-matched standard solution with R 2 ≥ 0.99. The limits of detection and limits of quantification were 0.3–1.5 and 0.9–4.7 μg/kg, respectively. The average recoveries of pesticides were 61.6–119.4% with relative standard deviations <16.1%. Furthermore, the method was applied successfully to analyse the pesticides in 15 pairs of organic and conventional vegetables. These results reflect the efficiency, reliability and robustness of the developed method.