Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation. (February 2019)

• Record Type: Journal Article
• Authors: Campos, Débora A.; Coscueta, Ezequiel R.; Valetti, Nadia Woitovich; Pastrana-Castro, Lorenzo M.; Teixeira, José A.; Picó, Guillermo A.; Pintado, Maria Manuela
• Is Part Of: Food hydrocolloids. Volume 87(2019)
• Page Start: 792
• Page End: 804
• Publication Date: 2019-02
• Subjects: Protein complex -- Natural crude juices -- Carrageenan -- Bromelain; BR bromelain -- Carr carrageenan -- LNPE N-α-carbobenzoxy-L-lysine-p-nitrophenyl ester (Z-L-Lys-ONp); Hydrocolloids -- Periodicals; Food additives -- Periodicals; Colloïdes -- Périodiques; Aliments -- Additifs -- Périodiques; Colloids; Food additives; Periodicals; Electronic journals; 664.06
• DOI: 10.1016/j.foodhyd.2018.09.009
• Languages: English
• ISSNs: 0268-005X

Abstract

Abstract: A simple method for bromelain extraction from industrial pineapple residues (stems and peels) was developed and optimized through factorial experimental design. The developed methodology, based on precipitation with carrageenan, represents an alternative to the use of organic solvents and inorganic salts (common industrial precipitation) and allows achieving extracts with high bromelain purity. High recovery yield – 80–90% - of active bromelain was obtained for both crude juices (stems and peels) making possible to obtain ca. 0.3 g of bromelain from 100 g of pineapple byproducts using a low polysaccharide concentration (0.2–0.3% w/v). Graphical abstract: Image 1 Highlights: Precipitation with carrageenan was applied to pineapple byproducts juices (stems and peels). Low polysaccharide concentration 0.2–0.3% (w/v) was successful in enzyme separation. Optimal extraction conditions were established through statistical modeling. Active bromelain was recovered with 80–90% of the yield of activity. Were obtain ca. 0.3 g of bromelain per each 100 g of byproducts.