Detoxification of mycotoxin patulin by the yeast Rhodotorula mucilaginosa. (February 2019)

• Record Type: Journal Article
• Authors: Li, Xiaohong; Tang, Hui; Yang, Chao; Meng, Xianghong; Liu, Bingjie
• Is Part Of: Food control. Volume 96(2019)
• Page Start: 47
• Page End: 52
• Publication Date: 2019-02
• Subjects: Rhodotorula mucilaginosa JM19 -- Patulin -- Mycotoxin -- Detoxification -- Degradatiosln compounds; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2018.08.029
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Patulin is a kind of mycotoxin, which occurs in food industry frequently and constitutes serious threats to human health. In this work, a strain of yeast with patulin degradation was screened, and identified as Rhodotorula mucilaginosa ( R. mucilaginosa JM19) by 26S rDNA D1/D2 region gene sequencing. Subsequently, the patulin detoxification process was analyzed by HPLC-UV. The results showed that patulin was degraded into desoxypatulinic acid by R. mucilaginosa JM19. In addition, patulin degrading capacity of R. mucilaginosa JM19 was strongly dependent on temperature, cell density and initial patulin concentration. Up to 90% of patulin was significantly reduced after 21 h in MES buffer at 35 °C when cell density of yeast was above 1 × 10 8 cells/L. The R. mucilaginosa JM19 is capable of detoxifying over 50% of initial patulin concentration at 100 μg/mL. The detoxification activity of R. mucilaginosa JM19 toward patulin would be a potential approach for the control of patulin contamination in food and raw materials. Highlights: Rhodotorula mucilaginosa was screened and identified by 26S rDNA D1/D2 sequences. The detoxification process of yeast was analyzed by HPLC. R.mucilaginosa could degrade patulin into desoxypatulinic acid. R.mucilaginosa was effective in detoxifying of patulin at 35 °C for 21 h.