A new HPTLC platformed luminescent biosensor system for facile screening of captan residue in fruits. (30th March 2020)

• Record Type: Journal Article
• Authors: Chen, Yisheng; Huang, Caihong; Hellmann, Bernhard; Jin, Zhengyu; Xu, Xueming; Xiao, Guosheng
• Is Part Of: Food chemistry. Volume 309(2020)
• Publication Date: 2020-03-30
• Subjects: Captan screening -- Fruits -- High performance thin-layer chromatography -- Luminescent biosensor; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.125691
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A HPTLC platformed biosensor system for facile screening of captan was proposed. A new bioluminescent bacterium suitable for silica gel layers was used. The established method was validated with six different fruits. The detectability of microbial biosensor displayed layer-dependence. Abstract: This study presented a HPTLC platformed luminescent biosensor system for screening captan residue. First, the potential bio-effects of layers materials on the detectability of a luminescent bacteria Photobacterium phosphoreum (ATCC 11040) as the sensor cell were assessed. From comparison, it was noteworthy that the combination of sensor cells with normal silica gel layer exclusively gave outstanding detectability (<10 ng/zone). On this basis, HPTLC mediated separation and biosensing was further optimized. Then, the obtained graphic results were digitally quantified via software processing, offering satisfactory selectivity, linearity ( R 2 = 0.9901 within 10–80 ng/zone) and sensitivity (0.5 mg/kg against MRLs ≥ 6 mg/kg). Additionally, the performance of the established method was validated with different fruits (recover rates 75–96%, RSD < 11.8%). Meanwhile, it was demonstrated that detectability of this hybrid system would be tuneable by altering the combination of bacteria strains and layer materials, which was meaningful to strengthen the usability of microbial biosensors.