Chlorogenic Acid Alleviates Hyperglycemia‐Induced Cardiac Fibrosis through Activation of the NO/cGMP/PKG Pathway in Cardiac Fibroblasts. Issue 2 (7th December 2020)

• Record Type: Journal Article
• Authors: Qin, Linhui; Zang, Mingxi; Xu, Yan; Zhao, Rongrong; Wang, Yating; Mi, Yang; Mei, Yingwu
• Is Part Of: Molecular nutrition & food research. Volume 65:Issue 2(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-12-07
• Subjects: cardiac fibrosis -- chlorogenic acid -- cyclic GMP -- diabetic cardiomyopathy -- endothelial nitric oxide synthase -- protein kinase G; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.202000810
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: Hyperglycemia‐induced cardiac fibrosis is one of the main causes of diabetic cardiomyopathy (DM). Chlorogenic acid (CGA) found in many foods has excellent hypoglycemic effectiveness, but it is not known whether CGA can improve DM by inhibiting cardiac fibrosis caused by hyperglycemia. Methods and Results: Type I diabetic mice are induced by streptozotocin, and after treatment with CGA for 12 weeks, cardiac functions and fibrosis are determined. CGA significantly attenuates hyperglycemia‐induced cardiac fibrosis and improves cardiac functions. The mechanism of CGA on fibrotic inhibition is further studied by immunofluorescence, western blot and RNA interference technology in vivo and in vitro. The results show CGA exerted its anti‐fibrotic effects through activating the cyclic GMP/protein kinase G pathway (cGMP/PKG) to block hyperglycemia‐induced nuclear translocation of p‐Smad2/3, and then inhibiting pro‐fibrotic gene expression in cardiac fibroblasts without depending on its hypoglycemic function. Moreover, the data also revealed that CGA increased cGMP level and activated PKG in cardiac fibroblasts by enhancing endothelial nitric oxide synthase (eNOS) activity and NO production. Conclusion: Besides lowering blood glucose, CGA also has an independent ability to inhibit cardiac fibrosis. Therefore, long‐term consumption of foods rich in CGA for diabetic patients will have great benefits to improve diabetic cardiomyopathy. Abstract : Chlorogenic acid is a polyphenol that can be found in many anti‐diabetic foods, such as tea and green coffee. It also shows remarkable anti‐fibrotic ability in diabetic heart through activating the endothelial nitric oxide synthase/NO/cyclic GMP/protein kinase G pathway and blocking the transforming growth factor‐β pathway.