The location of iron and zinc in grain of conventional and biofortified lines of sorghum. (September 2022)

• Record Type: Journal Article
• Authors: Gaddameedi, Anil; Sheraz, Sadia; Kumar, Ashok; Li, Kexue; Pellny, Till; Gupta, Rajeev; Wan, Yongfang; Moore, Katie L.; Shewry, Peter R.
• Is Part Of: Journal of cereal science. Volume 107(2022)
• Publication Date: 2022-09
• Subjects: Sorghum grain -- Biofortification -- NanoSIMS -- Localisation -- Iron -- Zinc; Grain -- Periodicals; Cereal products -- Periodicals; Céréales -- Périodiques; Produits céréaliers -- Périodiques; Cereal products; Grain; Periodicals; 664.705
• DOI: 10.1016/j.jcs.2022.103531
• Languages: English
• ISSNs: 0733-5210

Abstract

Abstract: Sorghum is an important source of dietary iron (Fe) and zinc (Zn) in parts of Africa and India, but there is a need to increase their concentrations to meet dietary requirements. Grains of a genetically biofortified sorghum line (Parbhani Shakti) had higher concentrations of Fe and Zn than a control line (M35-1). Analysis at the tissue level by histochemical staining and at the cellular level using NanoSIMS showed that both minerals are concentrated in the aleurone layer and in the scutellum of the embryo, with Zn also being concentrated in the embryonic axis. However, NanoSIMS showed that "hot spots" of 56 Fe + and 64 Zn + were also present in the sub-aleurone and starchy endosperm cells. Most of these hot spots also contained 31 P 16 O + indicating that the Fe and Zn are present as phytates, as in the aleurone and scutellum cells. Low concentrations of 56 Fe + and 64 Zn + were also observed in the protein matrix of these cells. Graphical abstract: Image 1 Highlights: A genetically biofortified sorghum line had high contents of Fe and Zn in grain. Mineral locations were determined at the cellular level using NanoSIMS. Both minerals are present as phytates in the aleurone layer and in the scutellum. Zn is also concentrated in the embryonic axis. "Hot spots" of Fe and Zn and P are present in the endosperm cells.