Arthritis biosensing: Aptamer-antibody-mediated identification of biomarkers by ELISA. (October 2022)

• Record Type: Journal Article
• Authors: Luo, Jian; Gopinath, Subash C.B.; Subramaniam, Sreeramanan; Wu, Zaifeng
• Is Part Of: Process biochemistry. Volume 121(2022)
• Page Start: 396
• Page End: 402
• Publication Date: 2022-10
• Subjects: Immunosensing -- Aptasensing -- Interleukin-6 -- Rheumatoid arthritis -- Dual probe; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2022.07.022
• Languages: English
• ISSNs: 1359-5113

Abstract

Abstract: Arthritis is a common disease in joints, causing tenderness, swelling and joint pain. Early identification of arthritis helps to prevent joint erosion, slow erosive disease progression, and achieve remission after treatment. Interleukin-6 (IL-6) is a cytokine biomarker that is overexpressed in response to inflammation, injury, and infection. Since elevation of IL-6 was found by researchers with arthritis patients, quantification of IL-6 helps for the early identification of arthritis. In this research, high affinity dual probed ELISA was introduced to identify IL-6 at low levels. On the gold nanourchin, the antibody and aptamer were attached and used as the detection probes to identify IL-6 on the ELISA surface. The dual probe was found to be highly sensitive and selective to IL-6 and bound more IL-6. The detection limit of IL-6 with the dual probe was calculated as 0.5 pg/mL by linear regression with an R 2 value of 0.9889. Furthermore, control experiments without detection probes or target and with the control protein IL-6 receptor did not show significant absorbance changes, confirming the specific detection of IL-6. This ELISA method with a dual probe identifies a lower abundance of IL-6 and helps to diagnose arthritis. Graphical Abstract: ga1 Highlights: Dual probed ELISA was to identify Interleukin-6 (IL-6) on gold nanourchin. Antibody and aptamer were attached to make highly sensitive & selective. The detection limit was as 0.5 pg/mL by a linear regression with R 2 of 0.9889. Control experiments did not show absorbance changes, confirms specific detection. ELISA strategy identifies the lower abundance of IL-6, helps to diagnose arthritis.