Effectively inhibiting the degradation of chitin during extraction from crustacean waste via a novel deep eutectic solvent aqueous solution. (October 2022)

• Record Type: Journal Article
• Authors: Wang, Yuehai; Yang, Yongqing; Wang, Rui; Zhu, Yanlin; Yang, Pengbo; Lin, Zhangnan; Wang, Zhihui; Cong, Wei
• Is Part Of: Process biochemistry. Volume 121(2022)
• Page Start: 142
• Page End: 151
• Publication Date: 2022-10
• Subjects: DESs deep eutectic solvents -- GA gluconic acid -- AA amino acids -- CS crab shell -- Asp aspartic acid -- Thr threonine -- Ser serine -- Glu glutamic acid -- Pro proline -- Gly glycine -- Ala alanine -- Cys cysteine -- Val valine -- Met methionine -- Ile isoleucine -- Leu leucine -- Phe phenylalanine -- Lys lysine -- His histidine -- Arg arginine -- Trp tryptophan -- Asn asparagine -- Gln glutamine -- FT-IR Fourier transform infrared spectroscopy -- XRD X-ray diffraction -- TGA thermal gravimetric analyser -- SEM scanning electron microscope -- NMR nuclear magnetic resonance; Crustacean waste -- Biopolymer chitin -- Deep eutectic solvents -- Inhibiting degradation -- High yield -- High molecular weight; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2022.06.029
• Languages: English
• ISSNs: 1359-5113

Abstract

Abstract: This study proposed a feasible route for effectively inhibiting degradation of chitin by introducing a novel low-viscosity gluconic acid-based deep eutectic solvents (DESs) aqueous solution. Under the optimal extraction conditions, a high-quality chitin with molecular weight of 3.75 × 10 5 Da, purity of 94.5 % and yield of 79.1 % was obtained from crab shell. The maximum yield and molecular weight of chitin extracted by DESs were 1.37 times and 3.02 times higher than traditional acid/alkali method, respectively. DESs showed satisfactory recycling performance and the chitin purity could still reach 82.6 % after reusing DESs four times. Mechanism analysis confirmed that introduction of amino acids enhanced the interactions in the DESs system. The main reason for inhibiting degradation of chitin was that the reagents mainly acted on calcium carbonate and protein, without appreciable effect on the structure of chitin. This study provided a green and effective utilization strategy for crustacean waste based on multifunctional DESs systems. Graphical Abstract: ga1 Highlights: Deep eutectic solvents with low viscosity and high solubility of chitin were used. Chitin degradation was effectively inhibited during extraction from crab shell. The maximum chitin yield was 1.37 times higher than traditional acid-base method. Molecular weight of chitin was 3.02 times higher than traditional acid-base method. Chitin purity could still reach 82.6 % after four cycles of deep eutectic solvents.