Isolation and identification of polyphenol monomers from celery leaves and their structure-antioxidant activity relationship. (October 2022)

• Record Type: Journal Article
• Authors: Wen, Chaoting; Song, Dandan; Zhuang, Linwu; Liu, Guoyan; Liang, Li; Zhang, Jixian; Liu, Xiaofang; Li, Youdong; Xu, Xin
• Is Part Of: Process biochemistry. Volume 121(2022)
• Page Start: 69
• Page End: 77
• Publication Date: 2022-10
• Subjects: Celery leaves -- Phenolic compounds -- Polyphenol monomer -- Antioxidant activity -- Structure-activity relationship; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2022.06.031
• Languages: English
• ISSNs: 1359-5113

Abstract

Abstract: This study aimed to isolate, purify and identify phenols from the celery leaves and further evaluate the antioxidant activities of the monomers in chemical system. Seven polyphenol monomers (a-g) were identified by HPLC-ESI-MS/MS after being separated by D101 macroporous resin, semi - preparative reversed-phase high-performance liquid chromatography (RP-HPLC) and sephadex LH-20. Interestingly, chrysoeriol 7-O-glucoside was identified from celery leaves by UV, IR, one dimensional nuclear magnetic resonance (NMR) and two-dimensional NMR for the first time. In addition, these polyphenol monomers manifested desirable DPPH scavenging capacity (EC50 : 39.55 ± 1.43–612 ± 8.45 μM) and ABTS+ scavenging capacity (EC50 : 32.12 ± 0.58–512 ± 25.12 μM), respectively. Among them, the polyphenol monomers (a and b) with two hydroxyl groups (-OH) on the B ring in flavone skeleton had the strongest antioxidant activities. Moreover, the analysis of the structure-activity relationship showed that the position and quantity of -OH on the B-ring and the methylation degree of 3-OH played an important role in regulating antioxidant activities. Graphical Abstract: ga1 Highlights: Celery leaf polyphenols were purified by using resin, RP-HPLC and sephadex LH-20. A new polyphenol monomer (Chrysoeriol 7-O-glucoside) was identified by using NMR. Antioxidant activity of purified polyphenol monomers were evaluated. Structure-activity relationship of purified polyphenol monomers were analyzed.