Heat, cold, acid, and bile salt induced differential proteomic responses of a novel potential probiotic Lactococcus garvieae C47 isolated from camel milk. (15th December 2022)

• Record Type: Journal Article
• Authors: Baig, Mohd Affan; Turner, Mark S.; Liu, Shao-Quan; Shah, Nagendra N.; Ayyash, Mutamed M.
• Is Part Of: Food chemistry. Volume 397(2022)
• Publication Date: 2022-12-15
• Subjects: Environmental stress -- Proteomics -- Lactic acid bacteria -- Nano-LC-MS/MS; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.133774
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Acid, bile, cold, heat stressed Lactococcus garvieae C47. 2D gel and nano-LC-MS-MS were employed to investigate the proteomic responses. The downregulated cyclic diguanylic acid can affect the motility and adhesion of bacteria. IGP synthase cyclase subunit can affect the biosynthesis of histidine. Overexpression of proteins could provide resistance to Lc. garvieae C47. Abstract: Probiotics encounter various stresses during food processing and digestion. This study evaluated the differential proteomic responses of a newly identified potential probiotic lactic acid bacteria, Lactococcus garvieae, isolated from camel milk. Lc. garvieae C47 was exposed to heat, cold, acid, and bile conditions, and stress-responsive proteins were identified. The proteomic analysis was done using 2D-IEF SDS PAGE and nano-LC-MS/MS. Out of 91 differentially expressed proteins, 20 upregulated and 27 downregulated proteins were shared among the stresses. The multivariate data analysis revealed abundance of elongation factor Ts (spot C42), uridine phosphorylase, fructose-bisphosphate aldolase, peptidase T, cobalt ECF transporter T component CbiQ, UDP- N -acetylmuramate-l -alanine ligase, uncharacterized protein, aspartokinase, chaperone protein DnaK, IGP synthase cyclase subunit, probable nicotinate-nucleotide adenylyltransferase, NADH-quinone oxidoreductase, holo-[acyl-carrier-protein] synthase, l -lactate dehydrogenase, and uncharacterized protein. The maximum number of differentially expressed proteins belonged to carbohydrate and protein metabolism, which indicates Lc. garvieae shifts towards growth and energy metabolism for resistance against stress conditions.