Development and validation of the one-step purification method coupled to LC-MS/MS for simultaneous determination of four aflatoxins in fermented tea. (30th August 2021)

• Record Type: Journal Article
• Authors: Zhou, Haiyan; Liu, Na; Yan, Zheng; Yu, Dianzhen; Wang, Lan; Wang, Kunbo; Wei, Xinlin; Wu, Aibo
• Is Part Of: Food chemistry. Volume 354(2021)
• Publication Date: 2021-08-30
• Subjects: Aflatoxins -- Tea -- Matrix effects -- One-step purification -- Liquid chromatography-tandem mass spectrometry; SPE Solid Phase Extraction -- DLLME Dispersive Liquid-Liquid Microextraction -- QuEChERS Quick Easy Cheap Effective Rugged and Safe -- IAC Immunoaffinity columns -- MFC Multifunctional Columns -- GO Graphene Oxide powder -- MWCNTs Multiwalled Carbon Nanotubes -- MWCNTs-COOH Carboxyl Multiwalled Carbon Nanotubes -- MWCNTs-OH Hydroxylated Multiwalled Carbon Nanotubes -- MWCNTs-NH2 Aminated Multiwalled carbon nanotubes -- PSA Primary and Secondary Amine -- SG Silica Gel -- HLB Hydrophilic-Lipophilic Balance -- D101, D101 macroporous adsorption resin -- AL-1 AL-1 weak polarity resin -- AL-A Alumina-A -- AL-N Alumina-N -- GCB graphitized carbon black -- NH2 Amino -- SRM selected reaction monitoring. RSDs, relative standard deviations -- LOQs limits of quantification -- LODs limits of detection -- LC-MS/MS liquid chromatography-tandem mass spectrometry; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.129497
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Schematic description of the extraction, cleaning, and analytical protocol of four aflatoxins determination in fermented tea samples. 10–20%, 50%, and 100% represent different degrees of fermentation. Highlight: A sensitive and accurate LC-MS/MS method for detecting aflatoxins in fermented tea was established. One-step purification has substantially enhanced the easiness and universality of approaches. MWCNTs-COOH was confirmed with the capability to clean-up complicated fermented tea matrices. Abstract: Aflatoxin B1 is the potential chemical contaminant of most concern during the production and storage of fermented tea. In this work, a simple, fast, sensitive, accurate, and inexpensive method has been developed and validated for the simultaneous detection of four aflatoxins in fermented tea based on a modified sample pretreatment method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Aflatoxins were extracted using acetonitrile and purified using mixed fillers (carboxyl multiwalled carbon nanotubes, hydrophilic-lipophilic balance, silica gel). Under optimum LC-MS conditions, the limits of quantification (LOQs) were 0.02–0.5 µg·kg −1 . Recoveries from aflatoxins-fortified tea samples (1–12 µg·kg −1 ) were in the range of 78.94–105.23% with relative standard deviations (RSDs) less than 18.20%. The proposed method was applied successfully to determine aflatoxin levels in fermented tea samples.