Creatine improves the flesh quality of Pacific white shrimp (Litopenaeus vannamei) reared in freshwater. (30th August 2021)

• Record Type: Journal Article
• Authors: Cheng, Xiaoli; Li, Meifeng; Leng, Xiangjun; Wen, Hua; Wu, Fan; Yu, Lijuan; Jiang, Ming; Lu, Xing; Gao, Weihua; Zhang, Wenbing; Tian, Juan
• Is Part Of: Food chemistry. Volume 354(2021)
• Publication Date: 2021-08-30
• Subjects: Litopenaeus vannamei -- Creatine -- Flesh quality -- Myofiber -- Amino acid; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.129498
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Dietary creatine improved the flesh quality of L. vannamei . Diet with 8.28 g kg −1 creatine increased muscular hardness and chewiness. Creatine decreased myofiber diameter and increased myofiber density. Creatine induced fast-to-slow fiber transition in muscle at the mRNA level. Creatine increased muscular protein and free amino acid contents. Abstract: Creatine improves flesh quality on mammalian but studies on crustaceans are scarce. In the present study, diets with six levels of creatine (1.23, 2.58, 5.12, 8.28, 14.12, 24.49 g kg −1 diet) were hand-fed to juvenile Litopenaeus vannamei (IBW: 1.50 ± 0.02 g) reared in freshwater for 46 days. Results showed creatine supplementation did not affect the growth performance (FBW: 17.04 ± 1.28 g) or the content of guanidinoacetic acid in muscle and hepatopancreas whereas significantly increased muscular creatine content. Diet with 8.28 g kg −1 creatine significantly increased muscular hardness and chewiness by decreasing myofiber diameter and increasing myofiber density. Additionally, creatine downregulated the mRNA expression of fast sMyHC1, sMyHC2, sMyHC6a and upregulated slow sMyHC5 and sMyHC15 mRNA expression. Muscular protein, collagen, total amino acid and flavor amino acid contents increased with creatine supplementation. In conclusion, the diet with 8.28 g kg −1 creatine improved the flesh quality of L. vannamei .