Advance diversity of enzymatically modified arabinoxylan from wheat chaff. (1st March 2021)

• Record Type: Journal Article
• Authors: Đorđević, Tatjana; Milošević, Maja; Antov, Mirjana
• Is Part Of: Food chemistry. Volume 339(2021)
• Publication Date: 2021-03-01
• Subjects: Enzymatic hydrolysis -- Arabinoxylan hydrolysates -- Modified arabinoxylan -- Wheat chaff -- Chemometric analysis; beta-d-Xylopyranose (PubChem CID125409) -- d-arabinose (PubChem CID66308) -- Ferulic acid (PubChem CID445858) -- ABTS (PubChem CID5360881) -- DPPH (PubChem CID2735032) -- Starch (PubChem CID439341 ); ABTS 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) -- Af +FE synergistic action of arabinofuranosidase and feruloyl esterase -- Af arabinofuranosidase action -- AX arabinoxylan -- DPPH 2, 2, diphenyl-1-picrylhydrazyl -- HCA hierarchical cluster analysis -- HP-GPC Gel permeation chromatography -- PCA principal component analysis -- PDI polydispersity index -- Xyn xylanase action -- Xyn+Af synergistic action of xylanase and arabinofuranosidase -- Xyn+Af+FE synergistic action of xylanase, arabinofuranosidase and feruloyl esterase -- Xyn+FE synergistic action of xylanase and feruloyl esterase; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.128093
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Enzymatic modification of arabinoxylan diversified its properties for advance applications. Arabinoxylan (AX) viscosity was highly modified by synergistic action of enzymes. Xylanase in synergism with debranching enzymes improves biological activity of AX. Abstract: Hydrolysates of arabinoxylan extracted from wheat chaff were prepared using different enzymatic treatments with an emphasis on improvements in their anti-diabetic, antioxidant and functional characteristics. The extracted arabinoxylan was subjected to enzymatic hydrolysis using individual xylanase, arabinofuranosidase, and feruloyl esterase, and their combinations. In all obtained hydrolysates, peaks corresponding to molecular weight lower than 38 kDa were noticed, while non-hydrolysed arabinoxylan had only peaks corresponding to 580 and 38 kDa. Results indicated that applied enzymes could hydrolyse polymeric arabinoxylan while their synergistic actions successfully modified its structure reflecting in lowered viscosity. Besides, it has been observed that the synergistic actions of enzymes improved the biological activities of arabinoxylan more than twice. Chemometric classification analysis showed that synergistic enzymes' actions were predominantly responsible for the improvement of biological activities. It indicated that they might be a useful tool for diversification and enhancement of biological activities of arabinoxylan from wheat chaff.