Deciphering the intra- and inter-kingdom networks of microbiota in the pit mud of Chinese strong-flavor liquor. (1st August 2022)

• Record Type: Journal Article
• Authors: Zhao, Lina; Wu, Ying; Zhao, Yanli; Li, Xuan; Zhang, Meng; Li, Xin; Ma, Jinliang; Gu, Shaobin
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 165(2022)
• Publication Date: 2022-08-01
• Subjects: Co-occurrence networks -- Prokaryotic community -- Eukaryotic community -- Pit mud -- Illumina MiSeq sequencing; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2022.113703
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Microbes have central roles in the pit mud (PM) ecosystems and flavor formation, yet specific ecological interactions and keystone taxa among these taxa are largely unknown. Here, we used network analyses to explore the PM microbial intra- and inter-kingdom networks based on Illumina MiSeq sequencing data. The results showed that Firmicutes (mainly Caproiciproducens, Lactobacillus and Bacillus ), Ascomycota (mainly Thermoascus, Eurotium and Aspergillus ) and Euryarchaeota (mainly Methanobacterium, Methanosarcina, Methanobrevibacter and Methanocorpusculum ) were the most dominant taxa detected in PM microbiota. The complexity of PM bacterial networks was much higher than that of fungal and archaeal networks, the hubs were mainly derived from bacteria rather than fungi and archaea in each inter-kingdom network, and the positive correlations dominated in bacterial (>75%), fungal (>90%) and inter-kingdom (>80%) networks. In addition, Mantel test showed that PM properties, especially pH, humic acid, available P and organic matter, were closely correlated with compositional structure of microbial communities and their main ecological clusters. The findings will contribute to a better understanding of PM microbiome structure and the ecological rules guiding community assembly, and have important implications for manipulating microbial resources and controlling PM environmental factors for pit management and sustainable brewing. Graphical abstract: Image 1 Highlights: The intra- and inter-kingdom networks of the PM microbiota were studied. PM bacterial networks were more complex than fungal and archaeal networks. Most of the "keystone" taxa in the inter-kingdom networks belonged to bacteria. Positive interactions among members of the PM microbiota was common. The syntrophic interactions of bacteria were critical for maintaining PM ecosystem stability.