Phenolic profiling, biological activities and in silico studies of Acacia tortilis (Forssk.) Hayne ssp. raddiana extracts. (August 2020)

• Record Type: Journal Article
• Authors: Ziani, Borhane E.C.; Carocho, Marcio; Abreu, Rui M.V.; Bachari, Khaldoun; Alves, Maria José; Calhelha, Ricardo C.; Talhi, Oualid; Barros, Lillian; Ferreira, Isabel C.F.R.
• Is Part Of: Food bioscience. Volume 36(2020)
• Publication Date: 2020-08
• Subjects: Acacia tortilis -- Phenolic compounds -- Cytotoxicity -- Anti-inflammatory -- Antimicrobial -- Molecular docking; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2020.100616
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: To evaluate the phenolic profile and biological activity of the Algerian Sahara plant Acacia tortilis (Forssk.) Hayne ssp. raddiana decoction and 80% ethanolic extracts were studied. Chromatographic profiling indicated the presence of 36 phenolic compounds, including gallic acid esterified derivatives, galloylquinic derivatives and flavan-3-ols galloyl derivatives. Both extracts showed significant cytotoxic activity and a potent anti-inflammatory activity. They were effective against several multi-drug resistant bacteria, namely methicillin-resistant Staphylococcus aureus (MRSA). To understand the possible mechanism of action of MRSA inhibition activity, an in silico docking analysis was done using a virtual library of the 36 determined phenolic compounds against penicillin-binding protein (PBP2a), a protein known to be involved in MRSA resistance to β-lactam antibiotics. A. tortilis extracts showed interesting biological activities and the phenolic compounds found could be an interesting starting point for the development of cytotoxic and anti-inflammatory drugs and especially anti-MRSA antibiotics. Graphical abstract: Image 1 Highlights: Acacia tortilis decoction and ethanol extracts were characterized. Esterified gallic acids, galloylquinic acids and flavan-3-ols galloyl derivatives were found. Both extracts showed cytotoxic, anti-inflammatory and antibacterial activity. Docking predicted PBP2a as a potential protein target for the phenolic compounds.