Antarctic krill derived peptide as a nanocarrier of iron through the gastrointestinal tract. (August 2020)

• Record Type: Journal Article
• Authors: Wang, Tongtong; Lin, Songyi; Cui, Pengbo; Bao, Zhijie; Liu, Kexin; Jiang, Pengfei; Zhu, Beiwei; Sun, Na
• Is Part Of: Food bioscience. Volume 36(2020)
• Publication Date: 2020-08
• Subjects: Antarctic krill peptide -- Euphausia superba -- Iron binding sites -- Caco-2 cells -- Iron bio-delivery; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2020.100657
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: Antarctic krill ( Euphausia superba ) might have specific peptides that chelate iron ions and are iron carriers. In this study, iron-binding peptides were isolated from Antarctic krill using enzymatic (trypsin) hydrolysis and immobilized metal affinity chromatography (IMAC); the binding mode and surface morphology of Antarctic krill peptides (AKP)-iron complexes as well as their iron delivery behavior in the gastrointestinal tract were investigated. Results showed that the AKP bound 79.3% of available iron from the enzymatic hydrolysis for 180 min. The contents of negatively-charged amino acids (Asp and Glu) on the AKP showed a positive correlation (r = 0.80) with iron-binding activities (the data includes Asn and Gln), while positively-charged amino acids showed a negative correlation (r = −0.83). Moreover, His, Ser and Thr on the AKP were also involved in the iron-binding activities. Iron-binding peptides from Antarctic krill hydrolysates, isolated using IMAC-Fe 3+ and identified using HPLC-MS/MS, showed a mass of 712–2451 Da, and were enriched in Asp (Asn), Glu (Gln), His, Ser or Thr. The iron ions mainly bound to carboxyl groups of AKP, thus forming nano-scale, spherical particles. The iron nanocarriers formed by AKP showed a significant iron transport activity after simulated gastrointestinal digestion, as compared to FeSO4 ( P < 0.05). The results suggested that the AKP-iron nanocomposite has the potential to be a nutraceutical supplement for improving iron absorption.