Simultaneous screening and analysis of 155 veterinary drugs in livestock foods using ultra-high performance liquid chromatography tandem quadrupole linear-ion-trap mass spectrometry. (1st November 2022)

• Record Type: Journal Article
• Authors: Wang, Juanqiang; Zhao, Wentao; Guo, Wenping; Li, Yingying; Jiang, Rui; Li, Huichen; Wang, Shouwei; Li, Zhigang
• Is Part Of: Food chemistry. Volume 393(2022)
• Publication Date: 2022-11-01
• Subjects: Multi-residue analysis -- Livestock foods -- Ultra-high performance liquid chromatography tandemquadrupole linear-ion-trap mass spectrometry -- Veterinary drugs -- Qualitative screening -- Quantitative analysis; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.133260
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Simultaneous screening and quantification of 155 veterinary drugs was conducted by UHPLC-QTRAP-MS in the multiple reaction monitoring-information dependent acquisition-enhanced product ion (MRM-IDA- EPI ) mode. Satisfactory qualitative and quantitative validation parameters were provided. Qualitative data was comparing with UHPLC-Q-Exactive Orbitrap/MS. The developed method showed several positive findings in real samples. Abstract: Veterinary drugs are widely used to improve the health and growth of livestock. The supervision of these residues is necessary to ensure food safety. A high-throughput method based on Oasis PRiME HLB with solid phase extraction for simultaneous qualitative and quantitative analysis of 155 veterinary drugs in livestock foods was developed by the ultra-high performance liquid chromatography tandem quadrupole linear-ion-trap mass spectrometry (UHPLC-QTRAP-MS). The limits of detection and quantification ranged from 0.5 μg/kg to 5 μg/kg and 2 μg/kg to 20 μg/kg, respectively. For over 85% of the analytes, the recoveries were between 60% and 120%. The positive simulated samples perfectly matched with a purity fit value over 70% from the self-built library. The screening results of UHPLC-QTRAP-MS were almost consistent with UHPLC tandem quadrupole-exactive orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap/MS). The evaluated UHPLC-QTRAP-MS method was powerful and reliable for the screening and quantification of veterinary drugs in real samples.