Structural characterization of a novel marine polysaccharide from mussel and its antioxidant activity in RAW264.7 cells induced by H2O2. (June 2022)

• Record Type: Journal Article
• Authors: Xiang, Xing-Wei; Wang, Rui; Chen, Hui; Chen, Yu-Feng; Shen, Guo-Xin; Liu, Shu-Lai; Sun, Pei-Long; Chen, Lin
• Is Part Of: Food bioscience. Volume 47(2022)
• Publication Date: 2022-06
• Subjects: Mussel polysaccharide -- Structural characterization -- Antioxidant activity; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2022.101659
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: In this study, a new type of water-soluble polysaccharide was extracted and purified from mussels, and its structure and cellular antioxidant levels were studied. Mussel polysaccharide (MP 4.25 × 10 3 Da) is composed mainly of glucose, with a small amount of galactosamine hydrochloride, glucosamine hydrochloride, and galactose. MP shows characteristic polysaccharide bands in Fourier transform NMR and infrared spectroscopy. The main chain connection mode of MP is the glycosidic bond of →4)-α-D-Glcp-(1→, and the end group α-D-Glcp-(1→ and α-D-Glcp-(1→6) -α-D-Glcp-(1→pass→4, 6)-α-D-Glcp-(1→. The O-6 bond is connected to the main chain. Moreover, MP could protect RAW264.7 cells against H2 O2 -induced oxidative stress by increasing catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activities and reducing the content of malondialdehyde (MDA). This is expected to make MP an important member of the antioxidant family. Graphical abstract: Image 1 Highlights: MP is a novel type of polysaccharide with the structure of α-D glucan. The molecular weight of MP is 4.25ⅹ10 3 Da. MP can increase the antioxidant level of RAW264.7 cells induced by H2 O2.