Antioxidant components of Viburnum opulus L. determined by on-line HPLC–UV–ABTS radical scavenging and LC–UV–ESI-MS methods. (15th May 2015)

• Record Type: Journal Article
• Authors: Karaçelik, Ayça Aktaş; Küçük, Murat; İskefiyeli, Zeynep; Aydemir, Sezgin; De Smet, Seppe; Miserez, Bram; Sandra, Patrick
• Is Part Of: Food chemistry. Volume 175(2015)
• Page Start: 106
• Page End: 114
• Publication Date: 2015-05-15
• Subjects: Viburnum opulus -- Snowball -- Gilaburu -- On-line HPLC–ABTS/DPPH -- FRAP -- Polyphenols; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.11.085
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: We determined radical scavenging activity in seed and skin extracts and juice of V. opulus by on-line HPLC method. Chlorogenic acid is the main compound identified with LC–MS in juice of V. opulus . ABTS radical scavenging method has better characteristics over DPPH method in on-line HPLC applications. The extractions with different solvents help elucidate different compounds of differing polarities. The antioxidant activities of the seed extracts were higher than the skin extracts in V. opulus . Abstract: Antioxidant activity of the juice and seed and skin extracts prepared with methanol, acetonitrile, and water of Viburnum opulus L. grown in Eastern Black Sea Region were studied with an on-line HPLC–ABTS method and off-line antioxidant methods, among which a linear positive correlation was observed. The fruit extracts were analysed with the HPLC–UV method optimised with 14 standard phenolics. Identification of the phenolic components in the juice was made using an HPLC–UV–ESI-MS method. Nineteen phenolic compounds in juice were identified by comparing the retention times and mass spectra with those of the standards and the phenolics reported in the literature. The major peaks in the juice belonged to coumaroyl-quinic acid, chlorogenic acid, procyanidin B2, and procyanidin trimer. Quite different antioxidant composition profiles were obtained from the extracts with the solvents of different polarities. The antioxidant activities of the seed extracts were higher than those of the skin extracts in general.