A novel green extraction and analysis technique for the comprehensive characterization of mangiferin in different parts of the fresh mango fruit (Mangiferaindica). (1st April 2022)

• Record Type: Journal Article
• Authors: Ahmad, Rizwan; Aldholmi, Mohammed; Mostafa, Ahmed; Alqathama, Aljawharah; Aldarwish, Alaa; Abuhassan, Asma; Alateeq, Leena; Bubshait, Salem; Aljaber, Muath; Aldossary, Saeed
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 159(2022)
• Publication Date: 2022-04-01
• Subjects: Mangiferin -- ASE -- UPLC-MS/MS -- Solvent -- Origin; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2022.113176
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: The study reports a novel, green, and effective ASE (accelerated solvent extraction) technique with a green, fast, and sensitive method of UPLC-MS/MS for a comprehensive characterization of whole mango fruit (epi-, endo-, Mesocarp, seed) under solvent, temperature, and origin effect in order to determine the inter- and intra-variation of MG(Mangiferin)-amount in fresh mango fruit. The ASE-MD (method development) showed the highest extract yield and more MG-amount in H2 O at 80 °C (mesocarp fruit part). For MG-quantification, a fast and sensitive UPLC-MS/MS method (RT = 0.31/runtime = 1 min; m/z 421.10 → 81.00 amu) was developed. The UPLC-MS/MS-MV validated the method with high accuracy, precision, and r 2 = 0.998. The intra-variation for MG was: mesocarp > endocarp > epicarp > seed. The inter-variation (MG-amount (mg/10g) in 12-mango cultivars) based on the sum of MG-amount/12-mango fruits parts: mesocarp (12.42)> epicarp (10.92)> endocarp (10.47)> seed (8.70) whereas, based on the largest MG-amount/individual mango fruit it was: mesocarp (5/12 fruits)> endocarp (4/12 fruits)> epicarp (3/12 fruits)> seed (0/12). The statistical models for PCA, K-mean cluster, and Pearson's analysis revealed significant correlation ( P ≤ 0.005 ) for origin*solvent*temperature Vs MG-amount with a nonsignificant correlation for origin*MG-amount Vs extract yield. The ASE-UPLC-MS/MS comprehensively characterized inter-, and intra-variation of MG in different parts of mango fruit. Highlights: Green and novel ASE-extraction and UHPLC-MSMS analysis method developed/validated. Solvents, temperatures, origin and fruit parts were correlated with MG-amount. Mesocarp revaled an average high amount of MG-amount in commercial mango sample. MG-amount in mango was independent of extract yield whereas dependent on sample origin. Solvents and temperatures were more correlated with MG-amount rather than extract yield.