Effects of electron beam irradiation pretreatment on the structural and functional properties of okara protein. (July 2022)

• Record Type: Journal Article
• Authors: Zhang, Meng; Feng, Xumei; Liang, Yaru; He, Mingyu; Geng, Mengjie; Huang, Yuyang; Teng, Fei; Li, Yang
• Is Part Of: Innovative food science & emerging technologies. Volume 79(2022)
• Publication Date: 2022-07
• Subjects: Okara protein -- electron beam irradiation -- Structural properties -- Functional properties; Food -- Biotechnology -- Periodicals; Food industry and trade -- Technological innovations -- Periodicals; Aliments -- Biotechnologie -- Périodiques; Food -- Biotechnology; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.ifset.2022.103049
• Languages: English
• ISSNs: 1466-8564

Abstract

Abstract: The structural and functional properties of okara protein pretreated with electron beam irradiation (EBI) at different doses (0–10 kGy) were investigated. The results of Fourier transform infrared spectroscopy spectra and fluorescence spectra indicated that EBI treatment decreased the α-helix and β-sheet proportion, contributing to a less compact tertiary conformation, thereby increased the fluorescence intensity with a red shift of λmax . Scanning electron microscopy images indicated that okara protein was fragmented into smaller pieces and the surface was uneven with pores after EBI treatment. The solubility, thermal stability and emulsification characteristics of okara protein were regulated by modifying its structure by EBI treatment. When the EBI treatment dose was 6 kGy, the protein had the highest solubility, as well as emulsification characteristics. Taken collectively, these results demonstrate that EBI can improve the functional properties of okara protein, which may widen the application of the protein. Highlights: Electron beam irradiation changed the advanced structure of okara protein. Okara protein surface integrity decreased in response to increasing irradiation dose. Okara protein exhibited better emulsification at irradiation dose of 6 kGy.