Chitosan-coated nanoliposomes for efficient delivery of betanin with enhanced stability and bioavailability. (November 2022)

• Record Type: Journal Article
• Authors: Liu, Shuang; Lian, Jiayi; Xu, Zhengming; Ning, Yue; Shi, Minyu; Zhao, Zhengang; Zhang, Zhanying
• Is Part Of: Food hydrocolloids. Volume 132(2022)
• Publication Date: 2022-11
• Subjects: Betanin -- Chitosan -- Nanoliposome -- Encapsulation -- Antioxidant; Hydrocolloids -- Periodicals; Food additives -- Periodicals; Colloïdes -- Périodiques; Aliments -- Additifs -- Périodiques; Colloids; Food additives; Periodicals; Electronic journals; 664.06
• DOI: 10.1016/j.foodhyd.2022.107871
• Languages: English
• ISSNs: 0268-005X

Abstract

Abstract: Betanin is a high-value health-promoting food ingredient. However, the bioavailability of betanin is constrained by its low stability. In this study, for the first time, betanin was stabilized and delivered using chitosan-coated nanoliposomes (CC-NLs). The CC-NLs were firstly synthesized and characterized. The results showed that increasing chitosan concentration from 0% to 1.0% increased particle size of CC-NLs from 156.4 nm to 217.2 nm. With the use of CC-NLs prepared with 0.6% chitosan, it was found that increasing pH from 1.5 to 9.0 reduced zeta potential of CC-NLs from 8.62 mv to-9.65 mv while the average size of CC-NLs of 221.40 nm peaked at pH 5.5. In aqueous solution, 29.0% of loaded betanin was released from NLs compared to 15.8% from CC-NLs. The in vitro digestion analysis indicated that uncoated NLs and CC-NLs had similar stabilities and relatively stable in oral and gastric fluids. However, in intestinal fluid, the average size of both NLs and CC-NLs were significantly reduced due to bile salt and trypsin. Furthermore, in vitro antioxidant activity assay results showed that betanin delivered by CC-NLs had the highest ORAC and PSC values. This study demonstrated that betanin could be efficiently delivered by CC-NLs with improved stability and bioavailability. The information generated from this study is very useful for the applications of betanin in functional food, pharmaceutical and cosmetics industry. Graphical abstract: Image 1 Highlights: Betanin was succussfully loaded on chitosan-coated nanoliposomes (CC-NLs). Controlled release of betanin from CC-NLs was achieved. CC-NLs were stable in simulated salivary and gastric fluids and were digested in simulated intestinal fluid. Betanin delivered by CC-NLs had the highest in vitro and cellular antioxidant activities.